Invitrogen

TLR3 Monoclonal Antibody (40C1285.6)

TLR3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TLR3 Antibody (MA5-16184) in IHC (P)

Immunohistochemical analysis of TLR3 in Tissue section of mouse intestine. Samples were incubated in TLR3 monoclonal antibody (Product # MA5-16184) using a dilution of 1:500 followed by HRP-DAB detection and hematoxylin counterstaining. The representative image shows a punctate staining of the ER and endosomes in a subset of cells in Peyers patches (organized lymphoid nodules) in the tested section.

TLR3 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

MA5-16184

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫组化（石蜡） (IHC (P))

1:10-1:500

免疫细胞化学 (ICC/IF)

1:10-1:500

流式细胞分析 (Flow)

2-4 µg per million cells

免疫沉淀 (IP)

2 µg per million cells

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中和实验 (Neu)

Assay-Dependent

产品规格

种属反应

Dog, Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

40C1285.6

抗原

A synthetic peptide corresponding to amino acids 55-85 of human TLR3 was used as immunogen.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2537703

靶标信息

TLR3 (Toll-like receptor 3, CD283) is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from drosophila to humans and share structural and functional similarities. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR3 is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. TLR3 recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. Further, TLR3 may play a role in host defense against viruses, and the use of alternative polyadenylation sites to generate different length transcripts has been noted for the TLR3 gene. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. TLR3 is the only human TLR which does not utilize MyD88 as an adaptor molecule instead utilizing TRIF in a signaling path which results in downstream activation of IRF-3 and NF-kB. TLR3 activation leads not only to Type I interferon induction but also to other inflammatory cytokines which result in DC maturation. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。