Invitrogen

TLR9 Polyclonal Antibody

查看其他36个TLR9抗体

抗体检测数据 (4)

TLR9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TLR9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TLR9 Antibody (PA5-27258) in IHC (P)

Immunohistochemistry (Paraffin) analysis of TLR9 was performed in paraffin-embedded mouse spleen tissue using TLR9 Polyclonal Antibody (Product # PA5-27258) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

TLR9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TLR9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TLR9 Antibody in Western Blot (WB)

TLR9 Antibody in Western Blot (WB)

Product Image Gallery

TLR9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of TLR9 was performed in paraffin-embedded mouse spleen tissue using TLR9 Polyclonal Antibody (Product # PA5-27258) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

TLR9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human lung cancer, using TLR9 (Product # PA5-27258) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

TLR9 Antibody in Western Blot (WB) — Western Blot using TLR9 Polyclonal Antibody (Product # PA5-27258). Sample (30 µg of whole cell lysate). Lane A: Non-transfected 293T lysates. Lane B: TLR9 transfected 293T lysates . 7.5% SDS PAGE. TLR9 Polyclonal Antibody (Product # PA5-27258) diluted at 1:2,000.

TLR9 Antibody in Western Blot (WB) — Western Blot using TLR9 Polyclonal Antibody (Product # PA5-27258). Sample (30 µg of whole cell lysate). A: THP-1 . 7.5% SDS PAGE. TLR9 Polyclonal Antibody (Product # PA5-27258) diluted at 1:1,000.

产品信息

PA5-27258

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a region within amino acids 218 and 314 of Human TLR9

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.4 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2544734

产品详细信息

Recommended positive controls: THP-1, Recombinant TLR9 protein.

Predicted reactivity: Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD289; Toll-like receptor 9

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基因别名: CD289; TLR9; UNQ5798/PRO19605

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UniProt ID: (Human) Q9NR96, (Mouse) Q9EQU3

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Entrez Gene ID: (Human) 54106, (Mouse) 81897

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transmembrane signaling receptor activity interleukin-1 receptor binding 1-phosphatidylinositol-3-kinase activity siRNA binding protein binding unmethylated CpG binding

参与通路

microglial cell activation regulation of protein phosphorylation toll-like receptor signaling pathway response to molecule of bacterial origin immune system process regulation of dendritic cell cytokine production MyD88-dependent toll-like receptor signaling pathway inflammatory response immune response signal transduction I-kappaB phosphorylation response to virus positive regulation of autophagy positive regulation of gene expression maintenance of gastrointestinal epithelium negative regulation of NF-kappaB transcription factor activity tumor necrosis factor production negative regulation of interleukin-6 production negative regulation of interleukin-8 production positive regulation of chemokine production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-beta production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production negative regulation of toll-like receptor signaling pathway positive regulation of toll-like receptor signaling pathway toll-like receptor 9 signaling pathway positive regulation of NF-kappaB import into nucleus positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of JUN kinase activity positive regulation of interferon-gamma biosynthetic process innate immune response positive regulation of interferon-alpha biosynthetic process positive regulation of interferon-beta biosynthetic process positive regulation of transcription from RNA polymerase II promoter regulation of cytokine secretion regulation of inflammatory response positive regulation of inflammatory response defense response to Gram-negative bacterium regulation of B cell activation positive regulation of NF-kappaB transcription factor activity defense response to virus positive regulation of nitric-oxide synthase biosynthetic process negative regulation of calcium-transporting ATPase activity axonogenesis phosphatidylinositol-3-phosphate biosynthetic process defense response to bacterium positive regulation of JNK cascade