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TLR9 Antibody (PA5-46898) in Flow
Flow cytometry of TLR9 in Human CD123+ peripheral blood mononuclear cells (PBMCs). Samples were incubated in TLR9 polyclonal antibody (Product # PA5-46898) or isotype control antibody. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.
产品信息
PA5-46898
产品规格
种属反应
Human
宿主/亚型
Sheep
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived recombinant human TLR9 Asn64-Glu189
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
纯化类型
Antigen affinity chromatography
保存液
Buffered saline solution with BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2577052
产品详细信息
In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse TLR9 is observed, and less than 1% cross-reactivity with recombinant human (rh) TLR1, rhTLR2, rhTLR3, rhTLR4, rhTLR5, rhTLR7, rhTLR8, and rhTLR10 is observed.
靶标信息
The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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生物信息学
蛋白别名: CD289; Toll-like receptor 9
基因别名: CD289; TLR9; UNQ5798/PRO19605
UniProt ID: (Human) Q9NR96
Entrez Gene ID: (Human) 54106
toll-like receptor signaling pathway
response to molecule of bacterial origin
MyD88-dependent toll-like receptor signaling pathway
inflammatory response
I-kappaB phosphorylation
axonogenesis
positive regulation of gene expression
maintenance of gastrointestinal epithelium
negative regulation of NF-kappaB transcription factor activity
tumor necrosis factor production
negative regulation of interleukin-6 production
negative regulation of interleukin-8 production
positive regulation of chemokine production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-beta production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-18 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
negative regulation of toll-like receptor signaling pathway
positive regulation of toll-like receptor signaling pathway
toll-like receptor 9 signaling pathway
phosphatidylinositol-3-phosphate biosynthetic process
positive regulation of NF-kappaB import into nucleus
defense response to bacterium
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of JUN kinase activity
positive regulation of interferon-gamma biosynthetic process
innate immune response
positive regulation of interferon-alpha biosynthetic process
positive regulation of interferon-beta biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
regulation of cytokine secretion
positive regulation of inflammatory response
defense response to Gram-negative bacterium
positive regulation of NF-kappaB transcription factor activity
positive regulation of nitric-oxide synthase biosynthetic process
negative regulation of calcium-transporting ATPase activity
