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TMED9 Antibody (MA5-50607) in ICC/IF
Immunocytochemistry analysis of TMED9 in HepG2 cells. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were incubated with TMED9 monoclonal antibody (Product # MA5-50607) with a dilution of 1:100 in 1% BSA in PBST overnight at 4 . Followed by secondary antibody Goat Anti-Mouse IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA wa... View More
产品信息
MA5-50607
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
A9A8
抗原
Recombinant protein within Human TMED9 aa 31-220/235.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3093990
产品详细信息
Positive control: Hela cell lysate, THP-1 cell lysate, A549 cell lysate, MCF-7 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, mouse lung tissue lysate, HepG2 cell lysate, LO2 cell lysate, human liver tissue lysate, rat liver tissue lysate, mouse liver tissue.
Predicted band size: 27 kDa
Subcellular Location: Endoplasmic reticulum membrane, Golgi apparatus, Endoplasmic reticulum-Golgi intermediate compartment membrane.
靶标信息
TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Glycoprotein 25L2; GMP25; gp25L2 protein; p24 family protein alpha-2; p24alpha2; p25; Transmembrane emp24 domain-containing protein 9; transmembrane emp24 protein transport domain containing 9
基因别名: 2400003B06Rik; GMP25; GP25L2; HSGP25L2G; p24a2; p25; RGD1307627; TMED9
UniProt ID: (Human) Q9BVK6, (Rat) Q5I0E7, (Mouse) Q99KF1
Entrez Gene ID: (Human) 54732, (Rat) 361207, (Mouse) 67511
