Invitrogen

TNF alpha Monoclonal Antibody (MAb11), PE, eBioscience™

TNF alpha Antibody in Flow Cytometry (Flow)

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TNF alpha Antibody (12-7349-41) in Flow

iScience 2023 - Phf6 R274X mutation increases the proliferation of hematopoietic stem cells (A) Hematoxylin and eosin (H&E) staining of BM, spleen, thymus, and liver. (B) Wright-Giemsa staining of PB smears and BM cytospin. (C) White blood cell (WBC), red blood cell (RBC), platelet (PLT), and lymphocyte (Lym) counts in PB by routine blood tests from Phf6 fl-R274X/Y (n = 5) and Vav1-Cre;Phf6 fl-R274X/Y (n = 4). (D) FACS ana... View More

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产品信息

12-7349-41

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看28篇已发表文章 28篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

MAb11

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10668834

产品详细信息

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MAb11 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online.

MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

R274X-mutated Phf6 increased the self-renewal and skewed T cell differentiation of hematopoietic stem cells.

iScience

Lan Y,Yuan S,Guo T,Hou S,Zhao F,Yang W,Cao Y,Chu Y,Jiang E,Yuan W,Wang X

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Fri Jun 16 00:00:00 EDT 2023

TIMP-1 is a novel ligand of Amyloid Precursor Protein and triggers a proinflammatory phenotype in human monocytes.

The Journal of cell biology

Eckfeld C,Schoeps B,Häußler D,Frädrich J,Bayerl F,Böttcher JP,Knolle P,Heisz S,Prokopchuk O,Hauner H,Munkhbaatar E,Demir IE,Hermann CD,Krüger A

12-7349-41 was used in Flow cytometry/Cell sorting to find that TIMP-1 triggered glucose uptake and proinflammatory cytokine expression in human monocytes.

Mon Feb 06 00:00:00 EST 2023

LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma.

Cell death & disease

Li H,Yang Z,Yang X,Zhang F,Wang J,Wu Z,Wanyan C,Meng Q,Gao W,Yang X,Wei J

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Thu Feb 03 00:00:00 EST 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Mon Dec 12 00:00:00 EST 2022

Enhanced antibody responses in fully vaccinated individuals against pan-SARS-CoV-2 variants following Omicron breakthrough infection.

Cell reports. Medicine

Jeong HW,Kim SM,Jung MK,Noh JY,Yoo JS,Kim EH,Kim YI,Yu K,Jang SG,Gil J,Casel MA,Rare R,Choi JH,Kim HS,Kim JH,Um J,Kim C,Kim Y,Chin BS,Jung S,Choi JY,Song KH,Kim YD,Park JS,Song JY,Shin EC,Choi YK

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

Cancer genes disfavoring T cell immunity identified via integrated systems approach.

Cell reports

Kishton RJ,Patel SJ,Decker AE,Vodnala SK,Cam M,Yamamoto TN,Patel Y,Sukumar M,Yu Z,Ji M,Henning AN,Gurusamy D,Palmer DC,Stefanescu RA,Girvin AT,Lo W,Pasetto A,Malekzadeh P,Deniger DC,Wood KC,Sanjana NE,Restifo NP

12-7349-41 was used in Flow cytometry/Cell sorting to establish that BIRC2 limits antigen presentation and T cell recognition of tumor cells by suppressing IRF1 activity and provide evidence that BIRC2 inhibition in combination with Adoptive T cell therapies is an effective strategy to increase efficacy.

Tue Aug 02 00:00:00 EDT 2022

Baricitinib treatment resolves lower-airway macrophage inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

Cell

Hoang TN,Pino M,Boddapati AK,Viox EG,Starke CE,Upadhyay AA,Gumber S,Nekorchuk M,Busman-Sahay K,Strongin Z,Harper JL,Tharp GK,Pellegrini KL,Kirejczyk S,Zandi K,Tao S,Horton TR,Beagle EN,Mahar EA,Lee MYH,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Delmas OM,Wang S,Cooney KA,Sayegh MN,Wang L,Filev PD,Weiskopf D,Silvestri G,Waggoner J,Piantadosi A,Kasturi SP,Al-Shakhshir H,Ribeiro SP,Sekaly RP,Levit RD,Estes JD,Vanderford TH,Schinazi RF,Bosinger SE,Paiardini M

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Thu Jan 21 00:00:00 EST 2021

Membrane lymphotoxin-α₂β is a novel tumor necrosis factor (TNF) receptor 2 (TNFR2) agonist.

Cell death & disease

Kucka K,Lang I,Zhang T,Siegmund D,Medler J,Wajant H

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Tue Apr 06 00:00:00 EDT 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Development of an Inflammatory CD14⁺ Dendritic Cell Subset in Humanized Mice.

Frontiers in immunology

Iwabuchi R,Ide K,Terahara K,Wagatsuma R,Iwaki R,Matsunaga H,Tsunetsugu-Yokota Y,Takeyama H,Takahashi Y

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.

Cancer research communications

Ando M,Kondo T,Tomisato W,Ito M,Shichino S,Srirat T,Mise-Omata S,Nakagawara K,Yoshimura A

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Inhibition of Cholesterol Esterification Enzyme Enhances the Potency of Human Chimeric Antigen Receptor T Cells against Pancreatic Carcinoma.

Molecular therapy oncolytics

Zhao L,Liu Y,Zhao F,Jin Y,Feng J,Geng R,Sun J,Kang L,Yu L,Wei Y

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Fri Mar 27 00:00:00 EDT 2020

Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy.

Scientific reports

Lee J,Sheen JH,Lim O,Lee Y,Ryu J,Shin D,Kim YY,Kim M

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2020

Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis.

eLife

Trivedi S,Labuz D,Anderson CP,Araujo CV,Blair A,Middleton EA,Jensen O,Tran A,Mulvey MA,Campbell RA,Hale JS,Rondina MT,Leung DT

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Mon Nov 09 00:00:00 EST 2020

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

Heterogeneous Tumor-Immune Microenvironments among Differentially Growing Metastases in an Ovarian Cancer Patient.

Cell

Jiménez-Sánchez A,Memon D,Pourpe S,Veeraraghavan H,Li Y,Vargas HA,Gill MB,Park KJ,Zivanovic O,Konner J,Ricca J,Zamarin D,Walther T,Aghajanian C,Wolchok JD,Sala E,Merghoub T,Snyder A,Miller ML

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Thu Aug 24 00:00:00 EDT 2017

Renal Sodium Gradient Orchestrates a Dynamic Antibacterial Defense Zone.

Cell

Berry MR,Mathews RJ,Ferdinand JR,Jing C,Loudon KW,Wlodek E,Dennison TW,Kuper C,Neuhofer W,Clatworthy MR

12-7349-41 was used in Flow Cytometry to reveal a critical accessory role for the homeostatic function of a vital organ in optimizing tissue defense.

Thu Aug 24 00:00:00 EDT 2017

The distribution and function of human memory T cell subsets in lung cancer.

Immunologic research

Sheng SY,Gu Y,Lu CG,Zou JY,Hong H,Wang R

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Application of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population.

PloS one

Baguma R,Penn-Nicholson A,Smit E,Erasmus M,Day J,Makhethe L,de Kock M,Hughes EJ,van Rooyen M,Pienaar B,Stone L,Hanekom W,Brennan MJ,Wallis RS,Hatherill M,Scriba TJ

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Wed Oct 18 00:00:00 EDT 2017

A microfluidic platform for high-throughput multiplexed protein quantitation.

PloS one

Volpetti F,Garcia-Cordero J,Maerkl SJ

12-7349 was used in Antibody array to test a novel device for quantifying biomarkers in multiple samples by immunoassay.

Fri Apr 29 00:00:00 EDT 2016

The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors.

BMC hematology

Silveira AC,Santana MA,Ribeiro IG,Chaves DG,Martins-Filho OA

12-7349 was used in Flow cytometry/Cell sorting to understand immune response to FVIII in patients with HA who were either positive [HAα-FVIII(+)] or negative [HAα-FVIII(-)] for inhibitors.

Thu Feb 05 00:00:00 EST 2015

Depressive symptoms post hip fracture in older adults are associated with phenotypic and functional alterations in T cells.

Immunity & ageing : I & A

Duggal NA,Upton J,Phillips AC,Hampson P,Lord JM

12-7349 was used in Flow cytometry/Cell sorting to investigate the synergistic effects of hip fracture physical trauma and psychological depressive symptoms on the aged immune system.

Wed Jan 28 00:00:00 EST 2015

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Tue Aug 12 00:00:00 EDT 2014

Cell-mediated immune responses to respiratory syncytial virus infection: magnitude, kinetics, and correlates with morbidity and age.

Human vaccines & immunotherapeutics

Geevarghese B,Weinberg A

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Mon Apr 25 00:00:00 EDT 2016

Association of a NOD2 gene polymorphism and T-helper 17 cells with presumed ocular toxoplasmosis.

The Journal of infectious diseases

Dutra MS,Béla SR,Peixoto-Rangel AL,Fakiola M,Cruz AG,Gazzinelli A,Quites HF,Bahia-Oliveira LM,Peixe RG,Campos WR,Higino-Rocha AC,Miller NE,Blackwell JM,Antonelli LR,Gazzinelli RT

12-7349 was used in Flow cytometry/Cell sorting to suggest that NOD2 influences the production of IL-17A by CD4 T lymphocytes and might contribute to the development of ocular toxoplasmosis.

Tue Jan 01 00:00:00 EST 2013

Evaluation of suppressive and pro-resolving effects of EPA and DHA in human primary monocytes and T-helper cells.

Journal of lipid research

Jaudszus A,Gruen M,Watzl B,Ness C,Roth A,Lochner A,Barz D,Gabriel H,Rothe M,Jahreis G

12-7349 was used in Flow cytometry/Cell sorting to demonstrate that EPA and DHA do not generally affect immune cell functions in an inhibitory manner.

Mon Apr 01 00:00:00 EDT 2013

Functional OCT4-specific CD4⁺ and CD8⁺ T cells in healthy controls and ovarian cancer patients.

Oncoimmunology

Di J,Massuger LF,Duiveman-de Boer T,Zusterzeel PL,Figdor CG,Torensma R

Published figure using TNF alpha monoclonal antibody (Product # 12-7349-41) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

Respiratory syncytial virus G protein CX3C motif impairs human airway epithelial and immune cell responses.

Journal of virology

Chirkova T,Boyoglu-Barnum S,Gaston KA,Malik FM,Trau SP,Oomens AG,Anderson LJ

12-7349 was used in Flow cytometry/Cell sorting to elucidate the contribution of a conserved motif in the RSV G protein to the pathogenesis of the disease.

Sun Dec 01 00:00:00 EST 2013

Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4.

Infection and immunity

Chase JC,Celli J,Bosio CM

12-7349 was used in Flow cytometry/Cell sorting to show that type A F. tularensis interferes with the ability of dendritic cells to alert the host of infection both intra- and extracellularly.

Thu Jan 01 00:00:00 EST 2009

生物信息学

蛋白别名: APC1 protein; Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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基因别名: DIF; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F

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UniProt ID: (Human) P01375

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Entrez Gene ID: (Human) 7124

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功能

protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding

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