Invitrogen

TNF alpha Monoclonal Antibody (MAb11), PE-Cyanine7, eBioscience™

TNF alpha Antibody in Flow Cytometry (Flow)

图: 1 / 21

TNF alpha Antibody (25-7349-41) in Flow

iScience 2023 - Phf6 R274X mutation increases the proliferation of hematopoietic stem cells (A) Hematoxylin and eosin (H&E) staining of BM, spleen, thymus, and liver. (B) Wright-Giemsa staining of PB smears and BM cytospin. (C) White blood cell (WBC), red blood cell (RBC), platelet (PLT), and lymphocyte (Lym) counts in PB by routine blood tests from Phf6 fl-R274X/Y (n = 5) and Vav1-Cre;Phf6 fl-R274X/Y (n = 4). (D) FACS ana... View More

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产品信息

25-7349-41

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看33篇已发表文章 33篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

MAb11

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1257208

产品详细信息

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MAb11 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online.

This MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

R274X-mutated Phf6 increased the self-renewal and skewed T cell differentiation of hematopoietic stem cells.

iScience

Lan Y,Yuan S,Guo T,Hou S,Zhao F,Yang W,Cao Y,Chu Y,Jiang E,Yuan W,Wang X

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Fri Jun 16 00:00:00 EDT 2023

LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma.

Cell death & disease

Li H,Yang Z,Yang X,Zhang F,Wang J,Wu Z,Wanyan C,Meng Q,Gao W,Yang X,Wei J

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Thu Feb 03 00:00:00 EST 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Mon Dec 12 00:00:00 EST 2022

Enhanced antibody responses in fully vaccinated individuals against pan-SARS-CoV-2 variants following Omicron breakthrough infection.

Cell reports. Medicine

Jeong HW,Kim SM,Jung MK,Noh JY,Yoo JS,Kim EH,Kim YI,Yu K,Jang SG,Gil J,Casel MA,Rare R,Choi JH,Kim HS,Kim JH,Um J,Kim C,Kim Y,Chin BS,Jung S,Choi JY,Song KH,Kim YD,Park JS,Song JY,Shin EC,Choi YK

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

Baricitinib treatment resolves lower-airway macrophage inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

Cell

Hoang TN,Pino M,Boddapati AK,Viox EG,Starke CE,Upadhyay AA,Gumber S,Nekorchuk M,Busman-Sahay K,Strongin Z,Harper JL,Tharp GK,Pellegrini KL,Kirejczyk S,Zandi K,Tao S,Horton TR,Beagle EN,Mahar EA,Lee MYH,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Delmas OM,Wang S,Cooney KA,Sayegh MN,Wang L,Filev PD,Weiskopf D,Silvestri G,Waggoner J,Piantadosi A,Kasturi SP,Al-Shakhshir H,Ribeiro SP,Sekaly RP,Levit RD,Estes JD,Vanderford TH,Schinazi RF,Bosinger SE,Paiardini M

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Thu Jan 21 00:00:00 EST 2021

An AAV-based, room-temperature-stable, single-dose COVID-19 vaccine provides durable immunogenicity and protection in non-human primates.

Cell host & microbe

Zabaleta N,Dai W,Bhatt U,Hérate C,Maisonnasse P,Chichester JA,Sanmiguel J,Estelien R,Michalson KT,Diop C,Maciorowski D,Dereuddre-Bosquet N,Cavarelli M,Gallouët AS,Naninck T,Kahlaoui N,Lemaitre J,Qi W,Hudspeth E,Cucalon A,Dyer CD,Pampena MB,Knox JJ,LaRocque RC,Charles RC,Li D,Kim M,Sheridan A,Storm N,Johnson RI,Feldman J,Hauser BM,Contreras V,Marlin R,Tsong Fang RH,Chapon C,van der Werf S,Zinn E,Ryan A,Kobayashi DT,Chauhan R,McGlynn M,Ryan ET,Schmidt AG,Price B,Honko A,Griffiths A,Yaghmour S,Hodge R,Betts MR,Freeman MW,Wilson JM,Le Grand R,Vandenberghe LH

25-7349-41 was used in Flow Cytometry to show that AAVCOVID-1, an adeno-associated viral (AAV), spike-gene-based vaccine candidate demonstrates potent immunogenicity in mouse and non-human primates following a single injection and confers complete protection from SARS-CoV-2 challenge in macaques.

Wed Sep 08 00:00:00 EDT 2021

Membrane lymphotoxin-α₂β is a novel tumor necrosis factor (TNF) receptor 2 (TNFR2) agonist.

Cell death & disease

Kucka K,Lang I,Zhang T,Siegmund D,Medler J,Wajant H

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Tue Apr 06 00:00:00 EDT 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Development of an Inflammatory CD14⁺ Dendritic Cell Subset in Humanized Mice.

Frontiers in immunology

Iwabuchi R,Ide K,Terahara K,Wagatsuma R,Iwaki R,Matsunaga H,Tsunetsugu-Yokota Y,Takeyama H,Takahashi Y

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Immunogenicity of an AAV-based, room-temperature stable, single dose COVID-19 vaccine in mice and non-human primates.

bioRxiv : the preprint server for biology

Zabaleta N,Dai W,Bhatt U,Chichester JA,Sanmiguel J,Estelien R,Michalson KT,Diop C,Maciorowski D,Qi W,Hudspeth E,Cucalon A,Dyer CD,Pampena MB,Knox JJ,LaRocque RC,Charles RC,Li D,Kim M,Sheridan A,Storm N,Johnson RI,Feldman J,Hauser BM,Zinn E,Ryan A,Kobayashi DT,Chauhan R,McGlynn M,Ryan ET,Schmidt AG,Price B,Honko A,Griffiths A,Yaghmour S,Hodge R,Betts MR,Freeman MW,Wilson JM,Vandenberghe LH

25-7349-41 was used in Flow Cytometry to show that this methodology holds as a very promising single dose, thermostable vaccine platform well-suited to address emerging pathogens on a global scale.

Tue Jan 19 00:00:00 EST 2021

Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.

Cancer research communications

Ando M,Kondo T,Tomisato W,Ito M,Shichino S,Srirat T,Mise-Omata S,Nakagawara K,Yoshimura A

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Inhibition of Cholesterol Esterification Enzyme Enhances the Potency of Human Chimeric Antigen Receptor T Cells against Pancreatic Carcinoma.

Molecular therapy oncolytics

Zhao L,Liu Y,Zhao F,Jin Y,Feng J,Geng R,Sun J,Kang L,Yu L,Wei Y

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Fri Mar 27 00:00:00 EDT 2020

Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy.

Scientific reports

Lee J,Sheen JH,Lim O,Lee Y,Ryu J,Shin D,Kim YY,Kim M

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2020

Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis.

eLife

Trivedi S,Labuz D,Anderson CP,Araujo CV,Blair A,Middleton EA,Jensen O,Tran A,Mulvey MA,Campbell RA,Hale JS,Rondina MT,Leung DT

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Mon Nov 09 00:00:00 EST 2020

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth.

JCI insight

Zhuang X,Maione F,Robinson J,Bentley M,Kaul B,Whitworth K,Jumbu N,Jinks E,Bystrom J,Gabriele P,Garibaldi E,Delmastro E,Nagy Z,Gilham D,Giraudo E,Bicknell R,Lee SP

25-7349-41 was used in Flow Cytometry to generate chimeric antigen receptors from 2 CLEC14A-specific antibodies and expressed them in T cells. In vitro studies demonstrated that, when exposed to their target antigen, these engineered T cells proliferate, release IFN-γ, and mediate cytotoxicity.

Fri Oct 02 00:00:00 EDT 2020

Vaccine induction of antibodies and tissue-resident CD8+ T cells enhances protection against mucosal SHIV-infection in young macaques.

JCI insight

Petitdemange C,Kasturi SP,Kozlowski PA,Nabi R,Quarnstrom CF,Reddy PBJ,Derdeyn CA,Spicer LM,Patel P,Legere T,Kovalenkov YO,Labranche CC,Villinger F,Tomai M,Vasilakos J,Haynes B,Kang CY,Gibbs JS,Yewdell JW,Barouch D,Wrammert J,Montefiori D,Hunter E,Amara RR,Masopust D,Pulendran B

25-7349-41 was used in Flow Cytometry to demonstrate that vaccination strategies that induce both CD8+ T cell and antibody responses can confer enhanced protection against infection.

Thu Feb 21 00:00:00 EST 2019

Heterogeneous Tumor-Immune Microenvironments among Differentially Growing Metastases in an Ovarian Cancer Patient.

Cell

Jiménez-Sánchez A,Memon D,Pourpe S,Veeraraghavan H,Li Y,Vargas HA,Gill MB,Park KJ,Zivanovic O,Konner J,Ricca J,Zamarin D,Walther T,Aghajanian C,Wolchok JD,Sala E,Merghoub T,Snyder A,Miller ML

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Thu Aug 24 00:00:00 EDT 2017

The distribution and function of human memory T cell subsets in lung cancer.

Immunologic research

Sheng SY,Gu Y,Lu CG,Zou JY,Hong H,Wang R

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Application of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population.

PloS one

Baguma R,Penn-Nicholson A,Smit E,Erasmus M,Day J,Makhethe L,de Kock M,Hughes EJ,van Rooyen M,Pienaar B,Stone L,Hanekom W,Brennan MJ,Wallis RS,Hatherill M,Scriba TJ

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Wed Oct 18 00:00:00 EDT 2017

IL-21 and probiotic therapy improve Th17 frequencies, microbial translocation, and microbiome in ARV-treated, SIV-infected macaques.

Mucosal immunology

Ortiz AM,Klase ZA,DiNapoli SR,Vujkovic-Cvijin I,Carmack K,Perkins MR,Calantone N,Vinton CL,Riddick NE,Gallagher J,Klatt NR,McCune JM,Estes JD,Paiardini M,Brenchley JM

25-7349 was used in Flow cytometry/Cell sorting to investigate methods to promote Th17 cell recovery in individuals infected with HIV, showing that IL-21 and probiotic therapy improve Th17 frequencies, microbial translocation, and microbiome in antiretroviral-treated, SIV-infected macaques.

Tue Mar 01 00:00:00 EST 2016

Long-lasting multifunctional CD8⁺ T cell responses in end-stage melanoma patients can be induced by dendritic cell vaccination.

Oncoimmunology

Wimmers F,Aarntzen EH,Duiveman-deBoer T,Figdor CG,Jacobs JF,Tel J,de Vries IJ

25-7349 was used in Flow cytometry/Cell sorting to investigate the functionality of tumour-specific CD8(+) T cells in melanoma patients upon dendritic cell vaccination, showing long-lasting multifunctional responses in end-stage patients.

Sat Jan 29 00:00:00 EST 2022

Macrophages Are Phenotypically and Functionally Diverse across Tissues in Simian Immunodeficiency Virus-Infected and Uninfected Asian Macaques.

Journal of virology

Ortiz AM,DiNapoli SR,Brenchley JM

25-7349 was used in Flow cytometry/Cell sorting to demonstrate that macrophages from healthy animals exhibit phenotypic and functional heterogeneity across tissues and across a variety of stimuli.

Mon Jun 01 00:00:00 EDT 2015

Qualification of a whole blood intracellular cytokine staining assay to measure mycobacteria-specific CD4 and CD8 T cell immunity by flow cytometry.

Journal of immunological methods

Kagina BM,Mansoor N,Kpamegan EP,Penn-Nicholson A,Nemes E,Smit E,Gelderbloem S,Soares AP,Abel B,Keyser A,Sidibana M,Hughes JE,Kaplan G,Hussey GD,Hanekom WA,Scriba TJ

25-7349 was used in Flow cytometry/Cell sorting to qualify the intracellular cytokine staining process and to assess the effects of long-term cryopreservation.

Sun Feb 01 00:00:00 EST 2015

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Tue Aug 12 00:00:00 EDT 2014

Monocyte subsets in schistosomiasis patients with periportal fibrosis.

Mediators of inflammation

Fernandes JS,Araujo MI,Lopes DM,de Souza Rda P,Carvalho EM,Cardoso LS

25-7349 was used in Flow cytometry/Cell sorting to suggest that the three different monocyte populations participate in the immunopathogenesis of periportal fibrosis.

Mon Dec 15 00:00:00 EST 2014

Cell-mediated immune responses to respiratory syncytial virus infection: magnitude, kinetics, and correlates with morbidity and age.

Human vaccines & immunotherapeutics

Geevarghese B,Weinberg A

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-41) in Flow Cytometry

Mon Apr 25 00:00:00 EDT 2016

Functional OCT4-specific CD4⁺ and CD8⁺ T cells in healthy controls and ovarian cancer patients.

Oncoimmunology

Di J,Massuger LF,Duiveman-de Boer T,Zusterzeel PL,Figdor CG,Torensma R

25-7349 was used in Flow cytometry/Cell sorting to demonstrate the existence of anti-CSC specific T cells in ovarian cancer patients.

Wed May 01 00:00:00 EDT 2013

Cancer/testis antigens are novel targets of immunotherapy for adult T-cell leukemia/lymphoma.

Blood

Nishikawa H,Maeda Y,Ishida T,Gnjatic S,Sato E,Mori F,Sugiyama D,Ito A,Fukumori Y,Utsunomiya A,Inagaki H,Old LJ,Ueda R,Sakaguchi S

25-7349 was used in Flow cytometry/Cell sorting to investigate the possible expression of cancer/testis antigens by adult T-cell leukemia/lymphoma cells.

Thu Mar 29 00:00:00 EDT 2012

Brefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia trachomatis.

Journal of immunological methods

Vicetti Miguel RD,Maryak SA,Cherpes TL

25-7349 was used in Flow cytometry/Cell sorting to compare the ability of brefeldin A and monensin to concurrently trap 4 cytokines within peripheral blood mononuclear cells.

Wed Oct 31 00:00:00 EDT 2012

Differential expression of CD300a/c on human TH1 and TH17 cells.

BMC immunology

Simhadri VR,Mariano JL,Zhou Q,DeBell KE,Borrego F

25-7349 was used in Flow cytometry/Cell sorting to conclude that the CD300a/c receptors are differentially expressed on human TH1 and TH17 cells, and that their ligation is capable of modulating TCR mediated signals.

Wed Nov 02 00:00:00 EDT 2011

Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.

PloS one

Narayanan S,Silva R,Peruzzi G,Alvarez Y,Simhadri VR,Debell K,Coligan JE,Borrego F

25-7349 was used in Flow cytometry/Cell sorting to investigate a subset within the CD4 T cell population defined by expression of the immunomodulatory receptor CD300a.

Thu May 13 00:00:00 EDT 2010

GM-CSF DNA: an adjuvant for higher avidity IgG, rectal IgA, and increased protection against the acute phase of a SHIV-89.6P challenge by a DNA/MVA immunodeficiency virus vaccine.

Virology

Lai L,Vödrös D,Kozlowski PA,Montefiori DC,Wilson RL,Akerstrom VL,Chennareddi L,Yu T,Kannanganat S,Ofielu L,Villinger F,Wyatt LS,Moss B,Amara RR,Robinson HL

25734941 was used in flow cytometry to assess the effects of using GM-CSF DNA with the DNA prime for a simian-human immunodeficiency virus-89.6 vaccine

Wed Dec 05 00:00:00 EST 2007

生物信息学

蛋白别名: APC1 protein; Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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基因别名: DIF; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F

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UniProt ID: (Human) P01375

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Entrez Gene ID: (Human) 7124

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功能

protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding

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