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TRA-1-81 Antibody (41-1100) in ICC/IF
Immunofluorescence analysis of TRA 1-81 was performed using 90% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TRA-1-81 (cl. 26) Mouse Monoclonal Antibody (Product # 41-1100) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L)/IgM (L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugat... View More
产品信息
41-1100
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
cl.26
抗原
2102Ep cl.2A6 human embryonal carcinoma cells
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Wet ice (domestic); Dry ice (international)
RRID
AB_2533495
产品详细信息
TRA-1-81 recognizes an antigen of similar size detected as by TRA-1-60, GCTM2, K4, and K21, and all these antibodies detect similar but distinct patterns of expression on panels of tumor-derived cell lines. TRA-1-81 and TRA-1-60 epitopes are associated with distinct molecular species. TRA-1-81, TRA-1-60, GCTM2, and K21 antibodies all recognize distinct epitopes formed by modifications of a common keratan sulfate core molecule that is characteristically expressed by human EC cells. TRA-1-81, clone cl.26, likely recognizes the carbohydrate part of the molecule as TRA-1-60 activity is enhanced by neuraminidase.
This antibody is specific for the TRA-1-81 antigen. On western blots, it identifies the target band at ~200-400 kDa. Reactivity has been confirmed with human EC1003.7 and NTERA-2 cell lysates by western blotting, with smooth muscle cells in frozen normal human arterioles and arteries, intestine, lung, skin, and uterus, as well as epithelial cells of the mammary ducts, stomach, small and large intestine tissues by indirect immunofluorescence, and with lysates from 125I surface-labeled 2102Ep cl.2A6 human EC cells by immunoprecipitation.
靶标信息
TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: tra1-81; TRA180
