Invitrogen

TRAF6 Polyclonal Antibody

3个已发表图片
2 篇参考文献
查看其他16个TRAF6抗体
说明书
实验方案
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说明书
实验方案
技术支持

产品信息

PA5-29622

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:3,000
查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000
-

免疫细胞化学 (ICC/IF)

1:100-1:1,000
-

免疫沉淀 (IP)

1:100-1:500
-
产品规格

种属反应

Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 144 and 408 of Human TRAF6
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3.39 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2547097

产品详细信息

Recommended positive controls: A431, A549, HeLa, HepG2, NIH-3T3, BCL-1, PC-12, Rat2.

Predicted reactivity: Mouse (80%), Pig (83%), Rhesus Monkey (97%), Bovine (82%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Tumor necrosis factor (TNF) receptor associated factors (TRAFs) were initially discovered as adaptor proteins that link the TNF receptor superfamily to signaling pathways and are thus important regulators of cell death and cellular response to stress. TRAF proteins share a homology region that allows them to bind to cell receptors and other TRAF proteins, causing the activation of different signal cascades depending on the TRAFs involved. For example, TRAF2 and TRAF3 directly bind to the CD40, a NF receptor superfamily member involved in inducing B cell immunity, and are critical for NF-kappa-B activation in mouse B lymphocytes. TRAF2 along with TRAF6 has also been shown to be required for CD40 signaling in nonhemopoietic cells. TRAF2 also interacts with the TRFR superfamily member lymphotoxin-beta receptor (LTbetaR) in association with TRAF3 and the apoptosis inhibitors cIAP1 and Smac.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer; RING finger protein 85; RING-type E3 ubiquitin transferase TRAF6; TNF receptor-associated factor 6; TNF receptor-associated factor 6, E3 ubiquitin protein ligase

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基因别名: 2310003F17Rik; AI851288; C630032O20Rik; MGC:3310; RNF85; TRAF6

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UniProt ID: (Human) Q9Y4K3, (Rat) B5DF45, (Mouse) P70196

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Entrez Gene ID: (Human) 7189, (Rat) 311245, (Mouse) 22034

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功能
ubiquitin-protein transferase activity tumor necrosis factor receptor binding protein binding zinc ion binding ligase activity protein kinase binding mitogen-activated protein kinase kinase kinase binding ubiquitin conjugating enzyme binding ubiquitin protein ligase binding thioesterase binding identical protein binding histone deacetylase binding protein kinase B binding protein N-terminus binding ubiquitin protein ligase activity scaffold/adaptor protein
参与通路
negative regulation of transcription from RNA polymerase II promoter activation of MAPK activity protein polyubiquitination ossification neural tube closure stimulatory C-type lectin receptor signaling pathway toll-like receptor signaling pathway positive regulation of T cell cytokine production MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway protein complex assembly I-kappaB kinase/NF-kappaB signaling activation of NF-kappaB-inducing kinase activity JNK cascade antigen processing and presentation of exogenous peptide antigen via MHC class II osteoclast differentiation membrane protein intracellular domain proteolysis positive regulation of protein ubiquitination positive regulation of lipopolysaccharide-mediated signaling pathway activation of protein kinase activity positive regulation of interleukin-2 production toll-like receptor 9 signaling pathway Fc-epsilon receptor signaling pathway T-helper 1 type immune response positive regulation of T cell proliferation odontogenesis of dentin-containing tooth myeloid dendritic cell differentiation positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of JUN kinase activity positive regulation of interleukin-12 biosynthetic process positive regulation of interleukin-6 biosynthetic process bone resorption positive regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell development positive regulation of smooth muscle cell proliferation T cell receptor signaling pathway positive regulation of T cell activation regulation of immunoglobulin secretion positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity protein autoubiquitination nucleotide-binding oligomerization domain containing signaling pathway interleukin-1-mediated signaling pathway protein K63-linked ubiquitination response to interleukin-1 cellular response to lipopolysaccharide positive regulation of transcription regulatory region DNA binding immune response signal transduction organ morphogenesis protein ubiquitination cytokine-mediated signaling pathway regulation of apoptotic process bone remodeling
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