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Invitrogen
TRIM32 Polyclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 7
TRIM32 Antibody (PA5-22316) in ICC/IF
TRIM32 Polyclonal Antibody detects TRIM32 protein at cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in ice-cold MeOH for 5 min. Green: TRIM32 protein stained by TRIM32 Polyclonal Antibody (Product # PA5-22316) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
产品信息
PA5-22316
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 1 and 289 of TRIM32 (Uniprot ID#Q13049)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_11157685
产品详细信息
Recommended positive controls: 293T, DDDDK-TRIM32-transfected 293T.
Predicted reactivity: Mouse (97%), Rat (98%), Cat (98%), Rhesus Monkey (99%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
TRIM32 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM32 localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 72 kDa Tat-interacting protein; bM468K2.2 (tripartite motif protein 32); E3 ubiquitin-protein ligase TRIM32; RING-type E3 ubiquitin transferase TRIM32; RP11-67K19.1; TAT-interactive protein, 72-KD; tripartite motif protein 32; tripartite motif-containing 32; Tripartite motif-containing protein 32; zinc finger protein 117; Zinc finger protein HT2A; zinc-finger protein HT2A
基因别名: 1810045E12Rik; 3f3; BBS11; HT2A; LGMD2H; TATIP; TRIM32; Zfp117
UniProt ID: (Human) Q13049, (Mouse) Q8CH72
Entrez Gene ID: (Human) 22954, (Mouse) 69807
transcription coactivator activity
RNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
myosin binding
Tat protein binding
translation initiation factor binding
ubiquitin binding
protein self-association
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
protein polyubiquitination
tissue homeostasis
actin ubiquitination
response to UV
protein ubiquitination
positive regulation of cell growth
positive regulation of cell migration
regulation of type I interferon production
negative regulation of viral transcription
response to tumor necrosis factor
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
fat cell differentiation
positive regulation of neuron differentiation
positive regulation of protein catabolic process
positive regulation of cell cycle
positive regulation of proteolysis
muscle cell cellular homeostasis
negative regulation of fibroblast proliferation
positive regulation of neurogenesis
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
positive regulation of striated muscle cell differentiation
axon development
negative regulation of viral release from host cell
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of interleukin-17-mediated signaling pathway
positive regulation of chemokine (C-C motif) ligand 20 production
positive regulation of cell motility
negative regulation of keratinocyte apoptotic process
