Invitrogen

Thymidylate Synthase Polyclonal Antibody

Thymidylate Synthase Antibody in Western Blot (WB)

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Thymidylate Synthase Antibody (PA5-22263) in WB

Western Blot using Thymidylate Synthase Polyclonal Antibody (Product # PA5-22263). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Thymidylate synthetase antibody [N3C3] Thymidylate Synthase Polyclonal Antibody (Product # PA5-22263) diluted at 1:1,000.

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产品信息

PA5-22263

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:3,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 122 and 313 of Thymidylate synthetase (Uniprot ID#P04818)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_11154298

产品详细信息

Recommended positive controls: A549, H1299, HCT116.

Predicted reactivity: Mouse (94%), Rat (95%), Zebrafish (88%), Xenopus laevis (85%), Dog (96%), Pig (96%), Rhesus Monkey (98%), Chimpanzee (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。