Invitrogen

Thymidylate Synthase Polyclonal Antibody

Thymidylate Synthase Antibody in Western Blot (WB)

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Thymidylate Synthase Antibody (PA5-81282) in WB

Western Blot using Thymidylate Synthase Polyclonal Antibody (Product # PA5-81282) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: HL60 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa... View More

Thymidylate Synthase Antibody in Immunoprecipitation (IP)

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产品信息

PA5-81282

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

免疫沉淀 (IP)

4-8 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to the C-terminus of the Human TYMS

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2788503

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human Thymidylate Synthase/TYMS.

靶标信息

Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

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