NeoBiotechnologies

Thymidylate Synthase Monoclonal Antibody (TYMS, 1884)

Thymidylate Synthase Antibody in Immunocytochemistry (ICC/IF)

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Thymidylate Synthase Antibody (7298-MSM4-P1ABX) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of PFA-fixed Ramos cells using Thymidylate Synthase Monoclonal Antibody (Product # 7298-MSM4-P1ABX) followed by goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red)..

Thymidylate Synthase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Thymidylate Synthase Antibody in Western Blot (WB)

Thymidylate Synthase Antibody in Flow Cytometry (Flow)

Thymidylate Synthase Antibody in Peptide array (ARRAY)

Thymidylate Synthase Antibody in SDS-PAGE (SDS-PAGE)

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产品信息

7298-MSM4-P1ABX

应用

建议稀释比

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免疫印迹 (WB)

1-2 µg/mL

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

免疫细胞化学 (ICC/IF)

1-2 µg/mL

流式细胞分析 (Flow)

1-2 µg/10^6 cells

肽阵列 (Array)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2c, kappa

分类

Monoclonal

类型

Antibody

克隆号

TYMS, 1884

抗原

Recombinant human thymidylate synthase protein fragment (around aa 60-174)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

靶标信息

Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。