Invitrogen

Tyrosine Hydroxylase Monoclonal Antibody (185)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1个已发表图片

10 篇参考文献

查看其他34个Tyrosine Hydroxylase抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Tyrosine Hydroxylase Antibody in Immunocytochemistry (ICC/IF)

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Tyrosine Hydroxylase Antibody (MA1-24654) in ICC/IF

Immunofluorescence analysis of Tyrosine Hydroxylase using of Tyrosine Hydroxylase using Tyrosine Hydroxylase Monoclonal Antibody (185) (Product # MA1-24654) was performed using 70% confluent log phase PC-12 cells treated with 5 µg/mL dexamethasone for 6 days. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 min... View More

Tyrosine Hydroxylase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Tyrosine Hydroxylase Antibody in Immunohistochemistry (IHC)

Tyrosine Hydroxylase Antibody in Western Blot (WB)

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产品信息

MA1-24654

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:25-1:50

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:50

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

185

抗原

Recombinant protein corresponding to the C-terminal portion of mouse Tyrosine Hydroxylase.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.24 mg/mL

保存液

tissue culture supernatant

内含物

15mM sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_795666

产品详细信息

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Microneurotrophin BNN27 Reduces Astrogliosis and Increases Density of Neurons and Implanted Neural Stem Cell-Derived Cells after Spinal Cord Injury.

Biomedicines

Georgelou K,Saridaki EA,Karali K,Papagiannaki A,Charalampopoulos I,Gravanis A,Tzeranis DS

MA1-24654 was used in Immunohistochemistry to provide evidence that small-molecule mimetics of endogenous neurotrophins can contribute to effective combinatorial treatments for SCI, by simultaneously regulating key events of SCI and supporting grafted cell therapies in the lesion site.

Thu Apr 13 00:00:00 EDT 2023

Somatostatin Neurons in the Mouse Pontine Nucleus Activate GABA_A Receptor Mediated Synaptic Currents in Locus Coeruleus Neurons.

Frontiers in synaptic neuroscience

Garcia DuBar S,Cosio D,Korthas H,Van Batavia JP,Zderic SA,Sahibzada N,Valentino RJ,Vicini S

MA1-24654 was used in Immunohistochemistry to include corticotropin-releasing factor (CRF+) expressing neurons in the BRN that control bladder contraction and somatostatin expressing (SST+) neurons whose role in this region has not been discerned.

Fri Oct 07 00:00:00 EDT 2022

Restoration of Noradrenergic Function in Parkinson's Disease Model Mice.

ASN neuro

Cui K,Yang F,Tufan T,Raza MU,Zhan Y,Fan Y,Zeng F,Brown RW,Price JB,Jones TC,Miller GW,Zhu MY

Published figure using Tyrosine Hydroxylase monoclonal antibody (Product # MA1-24654) in Immunocytochemistry

Tue Nov 30 00:00:00 EST 2021

Lack of Mucosal Cholinergic Innervation Is Associated With Increased Risk of Enterocolitis in Hirschsprung's Disease.

Cellular and molecular gastroenterology and hepatology

Keck S,Galati-Fournier V,Kym U,Moesch M,Usemann J,Müller I,Subotic U,Tharakan SJ,Krebs T,Stathopoulos E,Schmittenbecher P,Cholewa D,Romero P,Reingruber B,Bruder E,Group NS,Holland-Cunz S

MA1-24654 was used in Immunocytochemistry to study the impact of cholinergic signals Hirschsprung''s disease -associated enterocolitis.

Thu Jan 27 00:00:00 EST 2022

Membrane Trafficking Protein CDP138 Regulates Fat Browning and Insulin Sensitivity through Controlling Catecholamine Release.

Molecular and cellular biology

Zhou QL,Song Y,Huang CH,Huang JY,Gong Z,Liao Z,Sharma AG,Greene L,Deng JZ,Rigor MC,Xie X,Qi S,Ayala JE,Jiang ZY

MA1-24654 was used in Immunohistochemistry to report that mice without CDP138 develop obesity under normal chow diet (NCD) or high-fat diet (HFD) conditions.

Sun Apr 15 00:00:00 EDT 2018

An autopsy case of microencephaly, bizarre putaminal lesion, and cerebellar atrophy with heart and liver diseases.

Brain & development

Okoshi Y,Hayashi M,Kanda S,Yamamoto T

MA1-24654 was used in immunohistochemistry to report on an autopsy case displaying microencephaly, putaminal lesion, and cerebellar atrophy plus heart and liver disease

Mon Sep 01 00:00:00 EDT 2014

Melatonin alterations and brain acetylcholine lesions in sleep disorders in Cockayne syndrome.

Brain & development

Okoshi Y,Tanuma N,Miyata R,Hayashi M

MA1-24654 was used in immunohistochemistry to study Cockayne syndrome sleep disorders and the roles played by altered melatonin metabolism and loss of acetylcholine neurons

Sat Nov 01 00:00:00 EDT 2014

Lesions of acetylcholine neurons in refractory epilepsy.

ISRN neurology

Hayashi M,Nakajima K,Miyata R,Tanuma N,Kodama T

MA1-24654 was used in immunohistochemistry to study the presence of lesions in acetylcholinergic neurons in various types of refractory epilepsy

Thu Aug 30 00:00:00 EDT 2012

Intestinal epithelial stem/progenitor cells are controlled by mucosal afferent nerves.

PloS one

Lundgren O,Jodal M,Jansson M,Ryberg AT,Svensson L

MA1-24654 was used in immunohistochemistry to investigate the role of mucosal afferent nerves in the maintenance and differentiation of intestinal epithelial progenitor cells

Wed Feb 09 00:00:00 EST 2011

An autopsy case presenting repetitive hypoglycemia and unique cortical dysplasia.

Brain & development

Hayashi M,Hachiya Y,Arai N

MA1-24654 was used in immunohistochemistry to investigate a case with repetitive hypoglycemia and unique cortical dysplasia

Thu Apr 01 00:00:00 EDT 2010

生物信息学

蛋白别名: dystonia 14; EC 1.14.16.2; HGNC:11782; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase

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基因别名: DYT14; DYT5b; TH; The; TYH

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UniProt ID: (Human) P07101, (Mouse) P24529, (Rat) P04177

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Entrez Gene ID: (Human) 7054, (Mouse) 21823, (Rat) 25085

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