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Application
FIGURE: 1 / 2
UBE2A Antibody (PA5-29940) in ICC/IF
UBE2A Polyclonal Antibody detects UBE2A protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: UBE2A protein stained by UBE2A Polyclonal Antibody (Product # PA5-29940) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-29940
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 141 of Human UBE2A
Conjugate
Unconjugated
Form
Liquid
Concentration
0.3 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547414
Product Specific Information
Recommended positive controls: mouse brain.
Predicted reactivity: Mouse (100%), Rat (93%), Zebrafish (100%), Xenopus laevis (100%), Chicken (100%), Rhesus Monkey (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E2 ubiquitin-conjugating enzyme A; HR6A; RAD6 homolog A; Ubiquitin carrier protein A; ubiquitin conjugating enzyme E2A; Ubiquitin-conjugating enzyme E2 A; ubiquitin-conjugating enzyme E2A (RAD6 homolog); ubiquitin-conjugating enzyme E2A, RAD6 homolog; ubiquitin-conjugating enzyme HR6A; Ubiquitin-protein ligase A
Gene Aliases: HHR6A; HR6A; Mhr6a; MRXS30; MRXSN; RAD6A; UBC2; UBE2A
UniProt ID: (Human) P49459, (Mouse) Q9Z255
Entrez Gene ID: (Human) 7319, (Mouse) 22209
protein polyubiquitination
in utero embryonic development
DNA repair
postreplication repair
ubiquitin-dependent protein catabolic process
positive regulation of cell proliferation
response to UV
histone H2A ubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
protein autoubiquitination
maternal process involved in female pregnancy
protein K48-linked ubiquitination
protein K11-linked ubiquitination
cellular response to DNA damage stimulus
protein ubiquitination
histone ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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