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UBE2S Antibody (PA5-142029) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of UBE2S in U2OS cells using UBE2S Polyclonal Antibody (Product # PA5-142029). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
产品信息
PA5-142029
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide with sequence NSNVENLPPHIIR-C (aa 2-14).
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
TBS, pH 7.3, with 0.5% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2932692
产品详细信息
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
靶标信息
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E2 ubiquitin-conjugating enzyme S; E2-EPF; E2-EPF5; Ubiquitin carrier protein S; ubiquitin conjugating enzyme E2S; Ubiquitin-conjugating enzyme E2 S; ubiquitin-conjugating enzyme E2-24 kD; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-conjugating enzyme E2S; Ubiquitin-protein ligase S
基因别名: E2-EPF; E2EPF; EPF5; OK/SW-cl.73; UBE2S
UniProt ID: (Human) Q16763
Entrez Gene ID: (Human) 27338
cellular protein modification process
exit from mitosis
free ubiquitin chain polymerization
anaphase-promoting complex-dependent catabolic process
protein K29-linked ubiquitination
protein K27-linked ubiquitination
cell division
protein K63-linked ubiquitination
protein K11-linked ubiquitination
protein K6-linked ubiquitination
positive regulation of ubiquitin protein ligase activity
