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UNC5B Antibody (PA5-116108) in ICC/IF
Immunocytochemistry analysis of UNC5B in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116108) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-116108
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human UNC5B(Accession Q8IZJ1), corresponding to amino acid residues V387-L437.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900742
产品详细信息
Antibody detects endogenous levels of total UNC5B.
靶标信息
Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C. elegans; Netrin receptor UNC5B; p53-regulated receptor for death and life protein 1; p53RDL1; Protein unc-5 homolog 2; Protein unc-5 homolog B; transmembrane receptor Unc5H2; unc-5 homolog 2; unc-5 homolog B; unc5 homolog 2
基因别名: 6330415E02Rik; A630020F16; D10Bwg0792e; P53RDL1; UNC5B; UNC5H2; UNQ1883/PRO4326
UniProt ID: (Human) Q8IZJ1, (Mouse) Q8K1S3, (Rat) O08722
Entrez Gene ID: (Human) 219699, (Mouse) 107449, (Rat) 60630
angiogenesis
apoptotic process
signal transduction
positive regulation of phosphatidylinositol 3-kinase signaling
anterior/posterior axon guidance
negative regulation of neuron apoptotic process
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
multicellular organism development
axon guidance
netrin-activated signaling pathway
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
