Invitrogen

Ubiquilin 1 Polyclonal Antibody

Ubiquilin 1 Antibody in Western Blot (WB)

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Ubiquilin 1 Antibody (PA5-22174) in WB

Western blot analysis was performed on whole cell extract (30 µg lysate) of SH-SY5Y (Lane 1), U-87 mg (Lane 2), RAW 264.7 (Lane 3), A431 (Lane 4), HeLa (Lane 5), PC-3 (Lane 6), A549 (Lane 7) and tissue extract of Mouse Brain (Lane 8). The blot was probed with Anti-Ubiquilin 1 Polyclonal Antibody (Product # PA5-22174, 1:3,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 63 kDa band corresponding to Ubiqu... View More

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产品信息

PA5-22174

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment containing a sequence corresponding to a region within amino acids 64 and 345 (Q9UMX0) of Ubiquilin 1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_11157427

产品详细信息

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.

Predicted reactivity: Mouse (90%), Rat (90%), Sheep (96%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

PLIC1 is an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。