Invitrogen

Ubiquilin 2 Monoclonal Antibody (3D5E2)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

8个已发表图片

15 篇参考文献

查看其他11个Ubiquilin 2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Ubiquilin 2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 17

Ubiquilin 2 Antibody (37-7700) in ICC/IF

Immunofluorescence analysis of Ubiquilin / Dsk2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ubiquilin / Dsk2 (3D5E2) Mouse Monoclonal Antibody (Product # 37-7700) at 2µg/mL in 0.1% BSA and incuba... View More

Ubiquilin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ubiquilin 2 Antibody in Western Blot (WB)

Ubiquilin 2 Antibody in Immunohistochemistry (IHC)

Ubiquilin 2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

产品信息

37-7700

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看7篇已发表文章 7篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:100

免疫细胞化学 (ICC/IF)

2 µg/mL

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Human, Mouse, Yeast

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

3D5E2

抗原

Recombinant protein derived from an internal region of human ubiquilin.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533341

靶标信息

This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases; and thus, are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence.

F1000Research

McDowell I,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

Published figure using Ubiquilin 2 monoclonal antibody (Product # 37-7700) in Western Blot

Wed Jun 28 00:00:00 EDT 2023

The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence.

F1000Research

McDowell I,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

Published figure using Ubiquilin 2 monoclonal antibody (Product # 37-7700) in Western Blot

Wed Jun 28 00:00:00 EDT 2023

The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence.

F1000Research

McDowell I,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

Published figure using Ubiquilin 2 monoclonal antibody (Product # 37-7700) in Western Blot

Wed Jun 28 00:00:00 EDT 2023

The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence.

F1000Research

McDowell I,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

Published figure using Ubiquilin 2 monoclonal antibody (Product # 37-7700) in Western Blot

Wed Jun 28 00:00:00 EDT 2023

Human Ubiquilin 2 and TDP-43 copathology drives neurodegeneration in transgenic Caenorhabditis elegans.

G3 (Bethesda, Md.)

Saxton AD,Kraemer BC

37-7700 was used in Western Blot to show that this model of UBQLN2-mediated neurodegeneration may be useful for further mechanistic investigation into the molecular cascades driving neurodegeneration in ALS and ALS-FTLD.

Sat Aug 07 00:00:00 EDT 2021

Overexpression of UBQLN1 reduces neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.

Acta neuropathologica communications

Wang S,Tatman M,Monteiro MJ

37-7700 was used in Western Blot, Immunohistochemistry to test whether overexpression of UBQLN1 can alleviate disease in the P497S UBQLN2 mouse model of ALS/FTD by crossing transgenic (Tg) mouse lines expressing the two proteins and characterizing the resulting genotypes using a battery of pathologic and behavioral tests.

Wed Oct 07 00:00:00 EDT 2020

Overexpression of UBQLN1 reduces neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.

Acta neuropathologica communications

Wang S,Tatman M,Monteiro MJ

Wed Oct 07 00:00:00 EDT 2020

Overexpression of UBQLN1 reduces neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.

Acta neuropathologica communications

Wang S,Tatman M,Monteiro MJ

Wed Oct 07 00:00:00 EDT 2020

Mutation-dependent aggregation and toxicity in a Drosophila model for UBQLN2-associated ALS.

Human molecular genetics

Kim SH,Stiles SG,Feichtmeier JM,Ramesh N,Zhan L,Scalf MA,Smith LM,Pandey UB,Tibbetts RS

37-7700 was used in Immunoprecipitation to study the the normal functions of the proline-rich-repeat region and the impacts of amyotrophic lateral sclerosis-associated mutations.

Mon Jan 15 00:00:00 EST 2018

Signature changes in ubiquilin expression in the R6/2 mouse model of Huntington's disease.

Brain research

Safren N,Chang L,Dziki KM,Monteiro MJ

37-7700 was used in immunohistochemistry and western blot to evaluate the diagnostic significance of ubiquilin expression in Huntington disease

Mon Feb 09 00:00:00 EST 2015

Signature changes in ubiquilin expression in the R6/2 mouse model of Huntington's disease.

Brain research

Safren N,Chang L,Dziki KM,Monteiro MJ

37-7700 was used in immunohistochemistry and western blot to evaluate the diagnostic significance of ubiquilin expression in Huntington disease

Mon Feb 09 00:00:00 EST 2015

Ubiquilin-1 overexpression increases the lifespan and delays accumulation of Huntingtin aggregates in the R6/2 mouse model of Huntington's disease.

PloS one

Safren N,El Ayadi A,Chang L,Terrillion CE,Gould TD,Boehning DF,Monteiro MJ

37-7700 was used in immunohistochemistry and western blot to test if overexpression of ubiquilin-1 delays or prevents neurodegeneration in R6/2 mice

Mon Sep 29 00:00:00 EDT 2014

Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE).

Biochimica et biophysica acta

Pitcher DS,de Mattos-Shipley K,Wang Z,Tzortzis K,Goudevenou K,Flynn H,Bohn G,Rahemtulla A,Roberts I,Snijders AP,Karadimitris A,Kleijnen MF

37-7700 was used in western blot to identify the unique SDS-PAGE pattern of nuclear proteasomes.

Mon Dec 01 00:00:00 EST 2014

Studies of the role of ubiquitination in the interaction of ubiquilin with the loop and carboxyl terminal regions of presenilin-2.

Biochemistry

Ford DL,Monteiro MJ

Tue Jul 31 00:00:00 EDT 2007

Studies of the role of ubiquitination in the interaction of ubiquilin with the loop and carboxyl terminal regions of presenilin-2.

Biochemistry

Ford DL,Monteiro MJ

Tue Jul 31 00:00:00 EDT 2007

Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.

The Journal of biological chemistry

Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE

Fri Oct 27 00:00:00 EDT 2006

Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.

The Journal of biological chemistry

Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE

Fri Oct 27 00:00:00 EDT 2006

生物信息学

蛋白别名: Chap1; DSK2 homolog; Nedd4 binding protein 4; PLIC-2; Protein linking IAP with cytoskeleton 2; Ubiquilin-2; Ubiquitin-like product Chap1/Dsk2; Ubiquitin-related protein

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基因别名: ALS15; CHAP1; DSK2; HRIHFB2157; N4BP4; Plic-2; PLIC2; UBQLN2

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UniProt ID: (Human) Q9UHD9, (Mouse) Q9QZM0

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Entrez Gene ID: (Human) 29978, (Mouse) 54609

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功能

protein binding

参与通路

regulation of macroautophagy ER-associated ubiquitin-dependent protein catabolic process negative regulation of clathrin-mediated endocytosis positive regulation of ER-associated ubiquitin-dependent protein catabolic process negative regulation of G-protein coupled receptor internalization regulation of autophagosome assembly autophagy