Invitrogen

V-ATPase E1 Polyclonal Antibody

V-ATPase E1 Antibody in Immunocytochemistry (ICC/IF)

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V-ATPase E1 Antibody (PA5-29899) in ICC/IF

Immunofluorescence analysis of V-ATPase E1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-V-ATPase E1 Rabbit Polyclonal Antibody (Product # PA5-29899) at 1:200 dilution in 0.1% BSA, incubated at ... View More

V-ATPase E1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

V-ATPase E1 Antibody in Western Blot (WB)

V-ATPase E1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

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产品信息

PA5-29899

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 200 of Human ATP6V1E1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.41 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2547373

产品详细信息

Recommended positive controls: A549, H1299, HCT116, mouse brain, rat brain.

Predicted reactivity: Mouse (99%), Rat (98%), Xenopus laevis (82%), Chicken (87%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Rapid isolation and cryo-EM characterization of synaptic vesicles from mammalian brain.

FEBS open bio

Du K,Hu L,Wang P,Xue Y

PA5-29899 was used in Western Blotting to describe an improved and simple purification protocol for isolating SVs from mouse brain within 6 h, achieving a yield of approximately 0.4 mg of SVs per single brain.

Tue Nov 01 00:00:00 EDT 2022

Macrophages use a bet-hedging strategy for antimicrobial activity in phagolysosomal acidification.

The Journal of clinical investigation

Dragotakes Q,Stouffer KM,Fu MS,Sella Y,Youn C,Yoon OI,De Leon-Rodriguez CM,Freij JB,Bergman A,Casadevall A

PA5-29899 was used in Immunocytochemistry to analyze the dynamical distribution of phagolysosomal pH in murine and human macrophages that had ingested live or dead Cryptococcus neoformans cells, or inert beads.

Wed Jul 01 00:00:00 EDT 2020

Docosahexaenoic acid inhibits zymogen activation by suppressing vacuolar ATPase activation in cerulein-stimulated pancreatic acinar cells.

Genes & nutrition

Park Y,Ku L,Lim JW,Kim H

Published figure using V-ATPase E1 polyclonal antibody (Product # PA5-29899) in Western Blot

Mon Mar 23 00:00:00 EDT 2020

Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel.

The Journal of biological chemistry

Shi S,Montalbetti N,Wang X,Rush BM,Marciszyn AL,Baty CJ,Tan RJ,Carattino MD,Kleyman TR

PA5-29899 was used in Immunocytochemistry-Immunofluorescence to investigate the modulatory role of the PON family in in ion channel expression.

Fri Apr 10 00:00:00 EDT 2020

H⁺-ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney.

American journal of physiology. Renal physiology

Frische S,Chambrey R,Trepiccione F,Zamani R,Marcussen N,Alexander RT,Skjødt K,Svenningsen P,Dimke H

Published figure using V-ATPase E1 polyclonal antibody (Product # PA5-29899) in Immunocytochemistry

Sat Sep 01 00:00:00 EDT 2018

H⁺-ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney.

American journal of physiology. Renal physiology

Frische S,Chambrey R,Trepiccione F,Zamani R,Marcussen N,Alexander RT,Skjødt K,Svenningsen P,Dimke H

Published figure using V-ATPase E1 polyclonal antibody (Product # PA5-29899) in Immunocytochemistry

Sat Sep 01 00:00:00 EDT 2018

生物信息学

蛋白别名: ATPase, H+ transporting lysosomal (vacuolar proton pump), 32 kDa; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1; ATPase, H+ transporting, V1 subunit E; H(+)-ATPase E-like protein; H(+)-transporting two-sector ATPase, 31kDa subunit; H+ ATPase subunit E; H+-transporting ATP synthase chain E, vacuolar; lysosomal 31kDa; p31; V-ATPase 31 kDa subunit; V-ATPase subunit E 1; V-ATPase, subunit E; V-type proton ATPase subunit E 1; vacuolar H(+)-ATPase, E subunit; vacuolar H+ ATPase E1; Vacuolar proton pump subunit E 1; VATPase, H+ transporting, lysosomal V1 subunit E1

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基因别名: 2410029D23Rik; ATP6E; ATP6E2; ATP6V1E; ATP6V1E1; D6Ertd385e; E2; P31; Vma4

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UniProt ID: (Human) P36543, (Mouse) P50518, (Rat) Q6PCU2

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Entrez Gene ID: (Human) 529, (Mouse) 11973, (Rat) 297566

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功能

protein binding hydrogen-exporting ATPase activity, phosphorylative mechanism proton-transporting ATPase activity, rotational mechanism ATPase binding hydrolase activity

参与通路

transport ion transport ATP hydrolysis coupled proton transport proton transport metabolic process insulin receptor signaling pathway regulation of macroautophagy transferrin transport ion transmembrane transport phagosome acidification