Invitrogen

VCAM-1 (CD106) Recombinant Rabbit Monoclonal Antibody (SA05-04)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5个已发表图片

5 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

VCAM-1 (CD106) Antibody in Immunocytochemistry (ICC/IF)

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VCAM-1 (CD106) Antibody (MA5-31965) in ICC/IF

Immunofluorescence analysis of Vascular cell adhesion protein 1 was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with VCAM-1 (CD106) Recombinant Rabbit Monoclonal Antibody (SA05-04) (Product # MA5-31965)... View More

VCAM-1 (CD106) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VCAM-1 (CD106) Antibody in Western Blot (WB)

VCAM-1 (CD106) Antibody in Immunohistochemistry (IHC)

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产品信息

MA5-31965

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2000-1:5000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50

产品规格

种属反应

Human, Mouse, Rat, Zebrafish

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SA05-04

抗原

Synthetic peptide within Human VCAM1 aa 690-739

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2809259

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The Role of Palmitic Acid in the Co-Toxicity of Bacterial Metabolites to Endothelial Cells.

Vascular health and risk management

Choroszy M,Środa-Pomianek K,Wawrzyńska M,Chmielarz M,Bożemska E,Sobieszczańska B

Published figure using VCAM-1 (CD106) recombinant monoclonal antibody (Product # MA5-31965) in Western Blot

Tue Jul 11 00:00:00 EDT 2023

Silencing of lncRNA XIST impairs angiogenesis and exacerbates cerebral vascular injury after ischemic stroke.

Molecular therapy. Nucleic acids

Wang C,Dong J,Sun J,Huang S,Wu F,Zhang X,Pang D,Fu Y,Li L

MA5-31965 was used in Western Blot to evaluate the impact of long non-coding RNA X-inactive-specific transcript in acute cerebral ischemic stroke patients, middle cerebral artery occlusion mice, and oxygen-glucose deprivation and restoration brain endothelial cells.

Fri Dec 03 00:00:00 EST 2021

Silencing of lncRNA XIST impairs angiogenesis and exacerbates cerebral vascular injury after ischemic stroke.

Molecular therapy. Nucleic acids

Wang C,Dong J,Sun J,Huang S,Wu F,Zhang X,Pang D,Fu Y,Li L

Fri Dec 03 00:00:00 EST 2021

Complement Inhibition Alleviates Cholestatic Liver Injury Through Mediating Macrophage Infiltration and Function in Mice.

Frontiers in immunology

Guo Z,Chen J,Zeng Y,Wang Z,Yao M,Tomlinson S,Chen B,Yuan G,He S

MA5-31965 was used in Immunohistochemistry-frozen tissue to assess the role of complement in CLI and the therapeutic effect of the site-targeted complement inhibitor CR2-Crry in CLI.

Mon Feb 28 00:00:00 EST 2022

Complement Inhibition Alleviates Cholestatic Liver Injury Through Mediating Macrophage Infiltration and Function in Mice.

Frontiers in immunology

Guo Z,Chen J,Zeng Y,Wang Z,Yao M,Tomlinson S,Chen B,Yuan G,He S

MA5-31965 was used in Immunohistochemistry-frozen tissue to assess the role of complement in CLI and the therapeutic effect of the site-targeted complement inhibitor CR2-Crry in CLI.

Mon Feb 28 00:00:00 EST 2022

KLF4 alleviates cerebral vascular injury by ameliorating vascular endothelial inflammation and regulating tight junction protein expression following ischemic stroke.

Journal of neuroinflammation

Zhang X,Wang L,Han Z,Dong J,Pang D,Fu Y,Li L

MA5-31965 was used in Western Blotting to examine the correlation between serum levels of CAMs/KLF4 and infarct volume in acute CIS patients.

Tue Apr 07 00:00:00 EDT 2020

生物信息学

蛋白别名: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1; VCAM-1

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基因别名: CD106; INCAM-100; Vcam-1; VCAM1; VCAM1B

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UniProt ID: (Human) P19320, (Rat) P29534

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Entrez Gene ID: (Human) 7412, (Rat) 25361, (Mouse) 22329

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功能

integrin binding primary amine oxidase activity cell adhesion molecule binding

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