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VE-cadherin Antibody (PA5-19612) in ICC/IF
Immunofluorescent staining of MCF-7 cells using Product # PA5-19612, anti-VE Cadherin antibody. The cells were fixed with methanol (100%) for 5 minutes, permabilised with TBS-T (20mins), BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% PBS-Tween for 1 hour and exposed to the primary antibody at a concentration of 5 µg/mL overnight at 4C. The secondary antibody was a 448 fluorescence conjugated ... View More
产品信息
PA5-19612
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human,
Mammal,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human VE Cadherin.
偶联物
Unconjugated
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 1% BSA
内含物
0.02% sodium azide
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10979589
产品详细信息
Heat mediated antigen retrieval with Tris/EDTA buffer, pH 9.0, recommended prior to tissue staining.
This antibody is predicted to react with cow and pig based on sequence homology.
Store antibody at 4ºC for 1-2 weeks. For long-term storage, store at -20ºC.
靶标信息
VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 7B4 antigen; 7B4/cadherin-5; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; Vascular endothelial cadherin; VE-cadherin
基因别名: 7B4; AA408225; CD144; CDH5; VE-Cad; Vec; VEcad; VECD
UniProt ID: (Human) P33151, (Mouse) P55284
Entrez Gene ID: (Human) 1003, (Mouse) 12562
blood vessel maturation
cell-cell junction assembly
homophilic cell adhesion via plasma membrane adhesion molecules
transforming growth factor beta receptor signaling pathway
negative regulation of cell proliferation
single organismal cell-cell adhesion
adherens junction organization
positive regulation of establishment of endothelial barrier
regulation of establishment of cell polarity
cell adhesion
