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VGLUT2 Antibody (MA5-45509) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with VGLUT2 monoclonal antibody (Product # MA5-45509) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Syn... View More
产品信息
MA5-45509
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N29/29
抗原
Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2
偶联物
激发/发射光谱
482/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931963
产品详细信息
1 µg/mL of MA5-45509 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 60kDa.
This antibody was formerly sold as clone S29-29.
靶标信息
VGLU2 mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. This protein may also mediate the transport of inorganic phosphate.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: differentation-associated Na-dependent inorganic phosphate cotransporter; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; differentiation-associated Na-dependent inorganic phosphate cotransporter; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6; solute carrier family 17 (vesicular glutamate transporter), member 6; Solute carrier family 17 member 6; Vesicular glutamate transporter 2; VGlU2; VGluT2
基因别名: 2900073D12Rik; DNPI; SLC17A6; VGLUT2
UniProt ID: (Human) Q9P2U8, (Mouse) Q8BLE7, (Rat) Q9JI12
Entrez Gene ID: (Human) 57084, (Mouse) 140919, (Rat) 84487
