Invitrogen

VIP Polyclonal Antibody

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VIP Antibody (PA5-120606) in WB

Western blot analysis of VIP in extracts of various cell lines. Samples were incubated with VIP Polyclonal antibody (Product # PA5-120606) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

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产品信息

PA5-120606

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-169 of human VIP (NP_9194161)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.59 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2914178

产品详细信息

Positive test controls include: BxPC-3, U-87MG, SH-SY5Y, Mouse spleen, Mouse brain, Rat brain. The target is usually found in the following locations: Secreted.

Immunogen sequence: MDTRNKAQLL VLLTLLSVLF SQTSAWPLYR APSALRLGDR IPFEGANEPD QVSLKEDIDM LQNALAENDT PYYDVSRNAR HADGVFTSDF SKLLGQLSAK KYLESLMGKR VSNISEDPVP VKRHSDAVFT DNYTRLRKQM AVKKYLNSIL NGKRSSEGES PDFPEELEK

靶标信息

VIP encodes a protein that belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。