Invitrogen

VPS26B Polyclonal Antibody

VPS26B Antibody in Immunocytochemistry (ICC/IF)

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VPS26B Antibody (PA5-101953) in ICC/IF

Immunocytochemistry analysis of VPS26B in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with VPS26B polyclonal antibody (Product # PA5-101953) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

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产品信息

PA5-101953

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human VPS26B(Accession Q4G0F5), corresponding to amino acid residues G236-K286.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2851385

产品详细信息

Antibody detects endogenous levels of total VPS26B.

靶标信息

In mammals, there are two paralogues of yeast Vps26, VPS26A and VPS26B. VPS26 is a component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. VPS26A and VPS26B subunits define distinct retromer complexes. The retromer complex is important in recycling transmembrane receptors from endosomes to the trans-Golgi network (TGN).

仅用于科研。不用于诊断过程。未经明确授权不得转售。