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VPS4B Antibody (PA5-30316) in ICC/IF
VPS4B Polyclonal Antibody detects VPS4B protein at nucleus and endosome by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B protein stained by VPS4B Polyclonal Antibody (Product # PA5-30316) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
产品信息
PA5-30316
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 176 and 433 of Human VPS4B
偶联物
形式
Liquid
浓度
0.48 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2547790
产品详细信息
Recommended positive controls: 293T.
Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (88%), Xenopus laevis (88%), Chicken (89%), Rhesus Monkey (99%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cell migration-inducing 1; Cell migration-inducing gene 1 protein; Protein SKD1; Suppressor of K(+) transport growth defect 1; suppressor of K+ transport defect 1; vacuolar protein sorting 4B; Vacuolar protein sorting-associated protein 4B
基因别名: MIG1; SKD1; SKD1B; VPS4-2; VPS42; VPS4B
UniProt ID: (Human) O75351
Entrez Gene ID: (Human) 9525
cell separation after cytokinesis
potassium ion transport
autophagy
nucleus organization
vacuole organization
mitotic metaphase plate congression
regulation of centrosome duplication
positive regulation of G2/M transition of mitotic cell cycle
protein transport
endosomal transport
viral life cycle
viral release from host cell
cholesterol transport
cytoplasmic microtubule organization
endosome to lysosome transport via multivesicular body sorting pathway
response to lipid
multivesicular body assembly
viral budding via host ESCRT complex
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
positive regulation of viral process
regulation of viral process
protein depolymerization
negative regulation of cell death
late endosomal microautophagy
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
regulation of mitotic spindle assembly
positive regulation of viral release from host cell
negative regulation of exosomal secretion
positive regulation of exosomal secretion
positive regulation of centriole elongation
positive regulation of viral life cycle
ESCRT III complex disassembly
