Invitrogen

WFS1 Polyclonal Antibody

查看其他5个WFS1抗体

抗体检测数据 (2)

WFS1 Antibody in Immunocytochemistry (ICC/IF)

WFS1 Antibody in Immunocytochemistry (ICC/IF)

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WFS1 Antibody (PA5-116717) in ICC/IF

Immunofluorescence analysis of WFS1 in HeLa cells. Samples were incubated with WFS1 Polyclonal antibody (Product # PA5-116717) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

WFS1 Antibody in Immunocytochemistry (ICC/IF)

WFS1 Antibody in Western Blot (WB)

Product Image Gallery

WFS1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of WFS1 in HeLa cells. Samples were incubated with WFS1 Polyclonal antibody (Product # PA5-116717) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

WFS1 Antibody in Western Blot (WB) — Western blot analysis of WFS1 in various lysates. Samples were incubated with WFS1 Polyclonal antibody (Product # PA5-116717) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

产品信息

PA5-116717

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human WFS1 (NP_0059962)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.37 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2901348

产品详细信息

Positive Samples: MCF7, HeLa, Mouse heart, Mouse brain

Immunogen sequence: MDSNTAPLGP SCPQPPPAPQ PQARSRLNAT ASLEQERSER PRAPGPQAGP GPGVRDAAAP AEPQAQHTRS RERADGTGPT KGDMEIPFEE VLERAKAGDP KAQTEVGKHY LQLAGDTDEE LNSCTAVDWL VLAAKQGRRE AVKLLRRCLA DRRGITSENE REVRQLSSET DLERAVRKAA LVMYWKLNPK KKKQVAVAEL LENVGQVNEH DGGAQPGPVP KSLQKQRRML ERLVSSESKN YIALDDFVEI TKKYAKGVIP SSLFLQDDED DDELAGKSPE DLPLR

靶标信息

WFS1 is a novel component of Wolfram syndrome, a rare form of juvenile diabetes. WFS1 plays an important role in maintaining homeostasis of the endoplasmic reticulum (ER) in the pancreas. It is normally up-regulated during insulin secretion, whereas inactivation of the protein can cause ER stress. Chronic ER stress is a major involvement in Wolfram syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Wolfram antibody; Wolfram syndrome 1 (wolframin); Wolfram syndrome 1 homolog; Wolfram syndrome 1 protein homolog; Wolframin

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基因别名: AI481085; CTRCT41; WFRS; WFS; WFS1; WFSL; wolframin

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UniProt ID: (Human) O76024, (Mouse) P56695

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Entrez Gene ID: (Human) 7466, (Mouse) 22393, (Rat) 83725

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protein binding ubiquitin protein ligase binding activating transcription factor binding ATPase binding calmodulin binding calcium-dependent protein binding transporter activity

参与通路

negative regulation of transcription from RNA polymerase II promoter kidney development renal water homeostasis visual perception sensory perception of sound ER-associated ubiquitin-dependent protein catabolic process endoplasmic reticulum unfolded protein response positive regulation of protein ubiquitination endoplasmic reticulum calcium ion homeostasis response to endoplasmic reticulum stress glucose homeostasis negative regulation of programmed cell death negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of neuron apoptotic process positive regulation of growth protein stabilization neurological system process positive regulation of protein metabolic process regulation of cell cycle positive regulation of calcium ion transport calcium ion homeostasis negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of ATF6-mediated unfolded protein response negative regulation of type B pancreatic cell apoptotic process pancreas development olfactory behavior positive regulation of proteolysis polyubiquitinated misfolded protein transport ER overload response protein maturation by protein folding IRE1-mediated unfolded protein response positive regulation of adenylate cyclase activity