Invitrogen

WT1 Polyclonal Antibody

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抗体检测数据 (4)

WT1 Antibody in Immunocytochemistry (ICC/IF)

WT1 Antibody in Immunocytochemistry (ICC/IF)

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WT1 Antibody (PA5-116131) in ICC/IF

Immunocytochemistry analysis of WT1 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116131). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

WT1 Antibody in Immunocytochemistry (ICC/IF)

WT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

WT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

WT1 Antibody in Western Blot (WB)

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WT1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of WT1 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116131). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

WT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of WT1 in human kidney cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-116131) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

WT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of WT1 in mouse testis tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-116131) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

WT1 Antibody in Western Blot (WB) — Western blot analysis of WT1 in various samples (Lane 1: HeLa treated with blocking peptide Lane 2: HeLa; Lane 3: HepG2; Lane 4: 293). Samples were incubated with polyclonal antibody (Product # PA5-116131).

产品信息

PA5-116131

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human WT1(Accession P19544), corresponding to amino acid residues M1-L18.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2900765

产品详细信息

Antibody detects endogenous levels of total WT1.

靶标信息

The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Wilms tumor protein; Wilms tumor protein homolog; Wilms tumor protein isoform Ex4a(+); WT; WT33

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基因别名: AWT1; D630046I19Rik; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; Wt-1; WT1; WT33

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UniProt ID: (Human) P19544, (Rat) P49952

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Entrez Gene ID: (Human) 7490, (Mouse) 22431, (Rat) 24883

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transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding RNA binding protein binding zinc ion binding sequence-specific DNA binding transcription regulatory region DNA binding C2H2 zinc finger domain binding nucleic acid binding DNA binding double-stranded DNA binding metal ion binding C2H2 zinc finger transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter vasculogenesis ureteric bud development branching involved in ureteric bud morphogenesis kidney development regulation of organ formation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter germ cell development thorax and anterior abdomen determination heart development sex determination negative regulation of cell proliferation RNA splicing gonad development male gonad development tissue development negative regulation of cell growth adrenal gland development male genitalia development epithelial cell differentiation glomerulus development glomerular basement membrane development adrenal cortex formation camera-type eye development negative regulation of apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter mesenchymal to epithelial transition positive regulation of heart growth diaphragm development cardiac muscle cell fate commitment visceral serous pericardium development cellular response to cAMP cellular response to gonadotropin stimulus metanephric mesenchyme development glomerular visceral epithelial cell differentiation posterior mesonephric tubule development metanephric epithelium development metanephric S-shaped body morphogenesis negative regulation of metanephric glomerular mesangial cell proliferation positive regulation of male gonad development negative regulation of female gonad development positive regulation of metanephric ureteric bud development metanephros development mesonephros development transcription, DNA-templated apoptotic process spermatogenesis single fertilization regulation of gene expression negative regulation of translation sperm motility positive regulation of apoptotic process Sertoli cell development coronary vasculature development seminiferous tubule development negative regulation of mesenchymal cell apoptotic process involved in metanephros development