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Application
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YOD1 Antibody (MA5-55596) in WB
Western Blot analysis of various lysates using YOD1 Recombinant Monoclonal Antibody (Product # MA5-55596) at 1:2,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
MA5-55596
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
4F9O2
抗原
A synthetic peptide corresponding to a sequence within amino acids 249-348 of human YOD1.
偶联物
Unconjugated
形式
Liquid
浓度
1.18 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
内含物
0.05% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
产品详细信息
Immunogen Sequence: FGEDAGYTKR VLLIYDGIHY DPLQRNFPDP DTPPLTIFSS NDDIVLVQAL ELADEARRRR QFTDVNRFTL RCMVCQKGLT GQAEAREHAK ETGHTNFGEV
靶标信息
Deubiquitinating enzymes (DUBs) are proteases that specifically cleave ubiquitin linkages, negating the action of ubiquitin ligases. DUBA8 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DUBA-8; HIN-7; HIV-1-induced protease 7; HIV-induced protein-7-like protease; hsHIN7; OTU domain containing 2; OTU domain-containing protein 2; Ubiquitin thioesterase OTU1; YOD1 OTU deubiquinating enzyme 1 homolog; YOD1 OTU deubiquinating enzyme 1 homolog ( yeast); YOD1 OTU deubiquitinating enzyme 1 homologue
基因别名: 9930028C20Rik; DUBA8; HIN7; Hshin7; OTUD2; PRO0907; YOD1
UniProt ID: (Human) Q5VVQ6, (Rat) Q32Q05, (Mouse) Q8CB27
Entrez Gene ID: (Human) 55432, (Rat) 363982, (Mouse) 226418
thiol-dependent ubiquitin-specific protease activity
protein binding
ubiquitin protein ligase binding
metal ion binding
Lys63-specific deubiquitinase activity
ubiquitin-specific protease activity involved in negative regulation of retrograde protein transport, ER to cytosol
Lys48-specific deubiquitinase activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
cysteine protease
ER-associated ubiquitin-dependent protein catabolic process
endoplasmic reticulum unfolded protein response
protein K29-linked deubiquitination
protein K11-linked deubiquitination
protein K63-linked deubiquitination
protein K48-linked deubiquitination
negative regulation of retrograde protein transport, ER to cytosol
protein K27-linked deubiquitination
protein K33-linked deubiquitination
proteolysis
response to unfolded protein
