Invitrogen

ZNF306 Polyclonal Antibody

ZNF306 Antibody in Immunocytochemistry (ICC/IF)

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ZNF306 Antibody (PA5-102903) in ICC/IF

Immunocytochemistry analysis of ZNF306 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ZNF306 polyclonal antibody (Product # PA5-102903) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

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产品信息

PA5-102903

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human ZKSCAN3(Accession Q9BRR0), corresponding to amino acid residues G212-D262.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2852284

产品详细信息

Antibody detects endogenous levels of total ZKSCAN3.

靶标信息

ZNF306 is a protein coding gene. Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migration in various tumors and myelomas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: SCAN-KRAB-zinc finger gene 1; SCAN-KRAB-zinc finger protein; Zf47; Zinc finger and SCAN domain-containing protein 13; Zinc finger protein 306; Zinc finger protein 307; Zinc finger protein 309; Zinc finger protein 47 homolog; Zinc finger protein with KRAB and SCAN domains 3; zinc finger protein zfp47

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基因别名: 2810435N07Rik; AA408594; AI132359; dJ874C20.1; dJ874C20.1.; mszf35; Skz1; ZF47; Zfp-47; ZFP306; Zfp307; ZFP47; ZKSCAN3; ZNF306; ZNF309; ZSCAN13; ZSCAN35

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UniProt ID: (Human) Q9BRR0, (Mouse) Q91VW9

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Entrez Gene ID: (Human) 80317, (Mouse) 72739

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding metal ion binding nucleic acid binding C2H2 zinc finger transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated autophagy lysosome organization negative regulation of autophagy negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of cellular senescence regulation of transcription, DNA-templated transcription from RNA polymerase II promoter