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alpha Actinin 1/2/3/4 Antibody (PA5-114820) in ICC/IF
Immunocytochemistry analysis of alpha Actinin 1/2/3/4 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114820). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse Ig... View More
产品信息
PA5-114820
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ACTN1/2/3/4.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2899456
产品详细信息
Antibody detects endogenous levels of total ACTN1/2/3/4.
靶标信息
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: actinin 1 smooth muscle; actinin, alpha 1; actinin, alpha 2; actinin, alpha 3; alpha actinin 1a; alpha actinin 4; Alpha-actinin cytoskeletal isoform; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin skeletal muscle isoform 3; Alpha-actinin-1; Alpha-actinin-2; Alpha-actinin-3; Alpha-actinin-4; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; non-muscle alpha-actinin 1; Non-muscle alpha-actinin 4; Non-muscle alpha-actinin-1
基因别名: 1110008F24Rik; 3110023F10Rik; ACTININ-4; ACTN1; Actn1a; ACTN2; ACTN3; ACTN4; BDPLT15; C77391; CMD1AA; CMH23; FSGS; FSGS1
UniProt ID: (Human) P12814, (Human) P35609, (Human) Q08043, (Human) O43707, (Rat) Q9Z1P2, (Mouse) Q7TPR4, (Mouse) Q9JI91, (Mouse) O88990, (Mouse) P57780, (Rat) Q9QXQ0
Entrez Gene ID: (Human) 87, (Human) 88, (Human) 89, (Human) 81, (Rat) 81634, (Mouse) 109711, (Mouse) 11472, (Rat) 291245, (Mouse) 11474, (Rat) 171009, (Mouse) 60595, (Rat) 63836
double-stranded RNA binding
integrin binding
calcium ion binding
protein binding
vinculin binding
ligand-dependent nuclear receptor transcription coactivator activity
protein homodimerization activity
ion channel binding
actin filament binding
Ras guanyl-nucleotide exchange factor activity
phosphatidylinositol-4,5-bisphosphate binding
cytoskeletal protein binding
structural constituent of muscle
protein domain specific binding
LIM domain binding
thyroid hormone receptor coactivator activity
titin binding
identical protein binding
protein dimerization activity
FATZ binding
titin Z domain binding
actin binding
RNA polymerase II regulatory region sequence-specific DNA binding
nucleoside binding
chromatin DNA binding
nuclear hormone receptor binding
retinoic acid receptor binding
poly(A) RNA binding
protein N-terminus binding
metal ion binding
protein binding, bridging
protein complex binding
platelet degranulation
actin filament organization
platelet activation
platelet formation
platelet morphogenesis
regulation of apoptotic process
focal adhesion assembly
actin filament bundle assembly
negative regulation of cellular component movement
actin filament network formation
actin crosslink formation
platelet aggregation
regulation of nucleic acid-templated transcription
MAPK cascade
cell adhesion
microspike assembly
muscle filament sliding
regulation of membrane potential
negative regulation of potassium ion transport
positive regulation of potassium ion transport
positive regulation of GTPase activity
sarcomere organization
protein homotetramerization
actin filament uncapping
cardiac muscle cell development
phospholipase C-activating angiotensin-activated signaling pathway
establishment of protein localization to plasma membrane
negative regulation of potassium ion transmembrane transporter activity
positive regulation of potassium ion transmembrane transporter activity
negative regulation of protein localization to cell surface
positive regulation of receptor activity
positive regulation of endocytic recycling
positive regulation of cation channel activity
regulation of the force of skeletal muscle contraction
skeletal muscle atrophy
transition between fast and slow fiber
response to denervation involved in regulation of muscle adaptation
negative regulation of glycolytic process
positive regulation of skeletal muscle tissue growth
negative regulation of calcineurin-NFAT signaling cascade
negative regulation of oxidative phosphorylation
regulation of aerobic respiration
positive regulation of glucose catabolic process to lactate via pyruvate
cortical cytoskeleton organization
muscle contraction
transport
protein transport
peroxisome proliferator activated receptor signaling pathway
retinoic acid receptor signaling pathway
positive regulation of pinocytosis
positive regulation of cellular component movement
bicellular tight junction assembly
protein localization to bicellular tight junction
response to hypoxia
vesicle transport along actin filament
positive regulation of cell migration
positive regulation of sodium:proton antiporter activity
negative regulation of substrate adhesion-dependent cell spreading
positive regulation of NIK/NF-kappaB signaling
