Invitrogen

alpha Tubulin Monoclonal Antibody (DM1A), Alexa Fluor™ 488, eBioscience™

alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

alpha Tubulin Antibody (53-4502-82) in ICC/IF

Immunofluorescence analysis of alpha Tubulin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Tubulin Monoclonal Antibody (DM1A), Alexa Fluor 488, eBioscience™ (Product # 53-4502-80) at 5 µg/mL in ... View More

alpha Tubulin Antibody in Immunocytochemistry, Proximity Ligation Assay (PLA) (ICC/IF, PLA)

alpha Tubulin Antibody in Western Blot (WB)

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产品信息

53-4502-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看7篇已发表文章 7篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-Dependent

免疫细胞化学 (ICC/IF)

5 µg/mL

查看8篇已发表文章 8篇已发表文章

in situ PLA (PLA)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Dog, Human, Mouse, Non-human primate, Pig, Rat

已发表种属

Arthropod, Human, Mouse, Non-human primate

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

DM1A

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1210525

产品详细信息

Description: The monoclonal antibody DM1a recognizes the 50 kDa cytoskeletal protein alpha tubulin in a variety of species (human, mouse, rat, monkey, dog, pig, bovine, goat, hamster, guinea pig, kangaroo, amphibians, sea urchin, yeast and tobacco plants). Tubulin, the major component of microtubules, is a dimeric protein consisting of an alpha and beta subunit. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation resulting in assembly (polymerization) or disassembly (depolymerization). The dynamic nature of microtubules is most evident in the mitotic apparatus. The DM1a antibody recognizes the C-terminal end of the alpha tubulin isoform (amino acids 426-430).

Applications Reported: This DM1A antibody has been reported for use in immunohistochemical and immunocytochemical staining.

Applications Tested: This DM1A antibody has been tested by immunocytochemistry of adherent cells. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Failure to Thrive: Impaired BDNF Transport along the Cortical-Striatal Axis in Mouse Q140 Neurons of Huntington's Disease.

Biology

Maloney MT,Wang W,Bhowmick S,Millan I,Kapur M,Herrera N,Frost E,Zhang EY,Song S,Wang M,Park AB,Yao AY,Yang Y

53-4502-82 was used in Immunohistochemistry-immunofluorescence to demonstrate that quantum dots-brain-derived neurotrophic factor (BDNF) transport along the cortical-striatal axis involves the impairment of anterograde transport within axons of cortical neurons, and of retrograde transport within dendrites of striatal neurons.

Thu Jan 19 00:00:00 EST 2023

Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.

The Journal of cell biology

Zhang Y,Seemann J

Published figure using alpha Tubulin monoclonal antibody (Product # 53-4502-82) in Immunocytochemistry

Mon Jan 04 00:00:00 EST 2021

Targeting EML4-ALK gene fusion variant 3 in thyroid cancer.

Endocrine-related cancer

Aydemirli MD,van Eendenburg JDH,van Wezel T,Oosting J,Corver WE,Kapiteijn E,Morreau H

Published figure using alpha Tubulin monoclonal antibody (Product # 53-4502-82) in Western Blot

Tue May 11 00:00:00 EDT 2021

Targeted degradation of transcription factors by TRAFTACs: TRAnscription Factor TArgeting Chimeras.

Cell chemical biology

Samarasinghe KTG,Jaime-Figueroa S,Burgess M,Nalawansha DA,Dai K,Hu Z,Bebenek A,Holley SA,Crews CM

53-4502-82 was used in Western Blot to conclude that TRAFTAC technology is potentially a generalizable strategy to induce degradation of other transcription factors both in vitro and in vivo.

Thu May 20 00:00:00 EDT 2021

CenH3-Independent Kinetochore Assembly in Lepidoptera Requires CCAN, Including CENP-T.

Current biology : CB

Cortes-Silva N,Ulmer J,Kiuchi T,Hsieh E,Cornilleau G,Ladid I,Dingli F,Loew D,Katsuma S,Drinnenberg IA

53-4502-82 was used in Immunocytochemistry to study the composition and assembly of CenH3-deficient kinetochores of Lepidoptera (butterflies and moths).

Mon Feb 24 00:00:00 EST 2020

Giant ankyrin-B suppresses stochastic collateral axon branching through direct interaction with microtubules.

The Journal of cell biology

Chen K,Yang R,Li Y,Zhou JC,Zhang M

53-4502-82 was used in Immunocytochemistry to demonstrate that gAnkB suppresses axon collateral branching and prevents microtubule invasion of nascent axon branches through direct interaction with microtubules.

Mon Aug 03 00:00:00 EDT 2020

Giant ankyrin-B suppresses stochastic collateral axon branching through direct interaction with microtubules.

The Journal of cell biology

Chen K,Yang R,Li Y,Zhou JC,Zhang M

Mon Aug 03 00:00:00 EDT 2020

Loss of the Nuclear Protein RTF2 Enhances Influenza Virus Replication.

Journal of virology

Chia BS,Li B,Cui A,Eisenhaure T,Raychowdhury R,Lieb D,Hacohen N

Published figure using alpha Tubulin monoclonal antibody (Product # 53-4502-82) in Western Blot

Tue Oct 27 00:00:00 EDT 2020

PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.

Molecular neurobiology

Winkler SC,Shimobayashi E,Kapfhammer JP

Published figure using alpha Tubulin monoclonal antibody (Product # 53-4502-82) in Proximity Ligation Assay (PLA)

Tue Dec 01 00:00:00 EST 2020

PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.

Molecular neurobiology

Winkler SC,Shimobayashi E,Kapfhammer JP

Published figure using alpha Tubulin monoclonal antibody (Product # 53-4502-82) in Proximity Ligation Assay (PLA)

Tue Dec 01 00:00:00 EST 2020

Metabolic reprogramming related to whole-chromosome instability in models for Hürthle cell carcinoma.

Scientific reports

Addie RD,Kostidis S,Corver WE,Oosting J,Aminzadeh-Gohari S,Feichtinger RG,Kofler B,Aydemirli MD,Giera M,Morreau H

Published figure using alpha Tubulin monoclonal antibody (Product # 53-4502-82) in Western Blot

Fri Jun 12 00:00:00 EDT 2020

GATA factor-regulated solute carrier ensemble reveals a nucleoside transporter-dependent differentiation mechanism.

PLoS genetics

Zwifelhofer NM,Cai X,Liao R,Mao B,Conn DJ,Mehta C,Keles S,Xia Y,Bresnick EH

Published figure using alpha Tubulin monoclonal antibody (Product # 53-4502-82) in Western Blot

Tue Dec 01 00:00:00 EST 2020

RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.

Proceedings of the National Academy of Sciences of the United States of America

Gulbranson DR,Davis EM,Demmitt BA,Ouyang Y,Ye Y,Yu H,Shen J

Published figure using alpha Tubulin monoclonal antibody (Product # 53-4502-82) in Western Blot

Tue Sep 26 00:00:00 EDT 2017

Do mechanical strain and TNF-α interact to amplify pro-inflammatory cytokine production in human annulus fibrosus cells?

Journal of biomechanics

Likhitpanichkul M,Torre OM,Gruen J,Walter BA,Hecht AC,Iatridis JC

Published figure using alpha Tubulin monoclonal antibody (Product # 53-4502-82) in Immunocytochemistry

Tue May 03 00:00:00 EDT 2016

IP-10/CXCL10 induction in human pancreatic cancer stroma influences lymphocytes recruitment and correlates with poor survival.

Oncotarget

Lunardi S,Jamieson NB,Lim SY,Griffiths KL,Carvalho-Gaspar M,Al-Assar O,Yameen S,Carter RC,McKay CJ,Spoletini G,D'Ugo S,Silva MA,Sansom OJ,Janssen KP,Muschel RJ,Brunner TB

Published figure using alpha Tubulin monoclonal antibody (Product # 53-4502-82) in Western Blot

Sun Nov 30 00:00:00 EST 2014

Chikungunya virus nsP3 blocks stress granule assembly by recruitment of G3BP into cytoplasmic foci.

Journal of virology

Fros JJ,Domeradzka NE,Baggen J,Geertsema C,Flipse J,Vlak JM,Pijlman GP

Published figure using alpha Tubulin monoclonal antibody (Product # 53-4502-82) in Immunofluorescence

Mon Oct 01 00:00:00 EDT 2012

Chemotaxis of cell populations through confined spaces at single-cell resolution.

PloS one

Tong Z,Balzer EM,Dallas MR,Hung WC,Stebe KJ,Konstantopoulos K

53-4502 was used in Immunocytochemistry-immunoflourescence to develop a tool for the study of cellular chemotaxis at a single-cell resolution.

Mon Jul 09 00:00:00 EDT 2012

Asymmetric properties of the Chlamydomonas reinhardtii cytoskeleton direct rhodopsin photoreceptor localization.

The Journal of cell biology

Mittelmeier TM,Boyd JS,Lamb MR,Dieckmann CL

53-4502 was used in Immunofluorescence to study the relationship between the rhodopsin eyespot photoreceptor Channelrhodopsin 1 (ChR1) and acetylated microtubules in the unicellular green alga Chlamydomonas reinhardtii.

Mon May 16 00:00:00 EDT 2011

生物信息学

蛋白别名: alpha tubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; tubulin alpha 3; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog

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基因别名: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; RGD1309427; RGD1565476; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; Tubb2; Tubb2a; TUBB2B

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功能

GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding double-stranded RNA binding ubiquitin protein ligase binding molecular_function enzyme binding nucleotide binding protein heterodimerization activity tubulin

参与通路

G2/M transition of mitotic cell cycle cytoskeleton organization microtubule-based process cytoskeleton-dependent intracellular transport cell division cellular response to interleukin-4 microtubule cytoskeleton organization biological_process platelet degranulation neuron migration protein polymerization metabolic process

alpha Tubulin Monoclonal Antibody (DM1A), Alexa Fluor™ 488, eBioscience™

alpha Tubulin Antibody (53-4502-82) in ICC/IF

产品信息

53-4502-82

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

in situ PLA (PLA)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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