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beta Actin Antibody (PA5-72633) in WB
Western blot analysis of beta Actin in various lysates. Samples were incubated with beta Actin polyclonal antibody (Product # PA5-72633) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Zebrafish lysate; Lane 2: ZF4 whole cell lysate. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
产品信息
PA5-72633
应用
建议稀释比
已发表文章
产品规格
种属反应
Zebrafish
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH conjugated synthetic peptide between 188-215 amino acids from the Central region of DANRE actba
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2718487
靶标信息
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Actin, cytoplasmic 1; B-ACTZF; beta-actin; Beta-actin-1
基因别名: ACTB; actba; B-ACTZF; bact; bactin1; bactzf; wu:fd18f05
UniProt ID: (Zebrafish) Q7ZVI7
Entrez Gene ID: (Zebrafish) 57934
