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beta Actin Antibody (PA5-85271) in ICC/IF
beta Actin Polyclonal Antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: beta Actin stained by beta Actin Polyclonal Antibody (Product # PA5-85271) diluted at 1:500. Blue: Fluoroshield with DAPI .
产品信息
PA5-85271
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Chicken,
Fruit fly,
Horse,
Cat,
Hamster,
Human,
Mouse,
Pig,
Rat,
Xenopus,
Yeast
已发表种属
Dog
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin.
偶联物
形式
Liquid
浓度
1.74 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2792414
产品详细信息
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Japanese Medaka (98%), Xenopus laevis (99%), Dog (100%), Rabbit (99%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%).
Positive Control: 293T, A341, Jurkat, Raji, HeLa, HepG2, H1299, HCT116, MCF-7, NT2D1, PC-3, U87-MG, NIH-3T3, mouse brain, PC-12, Rat2, Rat brain, drosophila, Yeast lysate, Candida albicans (sc5314) whole cell lysate, Rice leaf, mouse aorta, H9C2, OKT3, mouse ileum tissue, mouse heart tissue, mouse skeletal muscle tissue, mouse brain tissue, human periodontal ligament.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: actin, beta; Actin, cytoplasmic 1; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; Cytoplasmic beta-actin; cytoskeletal beta actin; PS1TP5-binding protein 1
基因别名: ACTB; actin; Actx; Bact; Beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; XELAEV_18045052mg
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
structural constituent of cytoskeleton
protein binding
ATP binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
identical protein binding
nitric-oxide synthase binding
protein kinase binding
structural constituent of postsynaptic actin cytoskeleton
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
retina homeostasis
movement of cell or subcellular component
cytoskeleton organization
substantia nigra development
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
ATP-dependent chromatin remodeling
positive regulation of gene expression, epigenetic
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
postsynaptic actin cytoskeleton organization
axonogenesis
cell motility
retina development in camera-type eye
cellular response to electrical stimulus
