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beta Actin Loading Control Antibody (MA5-15739-BTIN) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), MDA-MB-231 (Lane 5), PC-12 (Lane 6), RSC96 (Lane 7), tissue extracts of Mouse Lung (Lane 8) and Rat stomach (Lane 9). The blot was probed with beta Actin Loading Control Monoclonal Antibody (BA3R), Biotin (Product # MA5-15739-BTIN, 1 µg/mL) and detected by chemiluminesce... View More
产品信息
MA5-15739-BTIN
产品规格
种属反应
Dog,
Chicken,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
已发表种属
Human,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
BA3R
抗原
Beta-actin N-terminal peptide.
偶联物
Biotin
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with proprietary stabilizer
内含物
0.02% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2537661
产品详细信息
MA5-15739-BTIN has been successfully used for Western blot and FACS applications.
靶标信息
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: actin, beta; Actin, cytoplasmic 1; Bact; actin; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1
基因别名: ACT-5; ACTB; actin; Actx; Bact; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1
UniProt ID: (Rabbit) P29751, (Human) P60709, (Chicken) P60706, (Dog) O18840, (Rat) P60711, (Mouse) P60710
Entrez Gene ID: (Rabbit) 100009272, (Human) 60, (Chicken) 396526, (Dog) 403580, (Rat) 81822, (Mouse) 11461
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
structural constituent of cytoskeleton
protein binding
ATP binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
identical protein binding
nitric-oxide synthase binding
protein kinase binding
structural constituent of postsynaptic actin cytoskeleton
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
retina homeostasis
movement of cell or subcellular component
cytoskeleton organization
substantia nigra development
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
ATP-dependent chromatin remodeling
positive regulation of gene expression, epigenetic
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
axonogenesis
cell motility
postsynaptic actin cytoskeleton organization
retina development in camera-type eye
cellular response to electrical stimulus
