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beta-Arrestin 1,2 Antibody (PA1-730) in IHC (P)
Immunohistochemistry analysis of pan Arrestin showing staining in the cytoplasm and nucleus of paraffin-treated human cerebellum tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol ... View More
产品信息
PA1-730
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Fruit fly,
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptide: C D(384) D I V F E D F A R L R L K(397)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2274371
产品详细信息
PA1-730 detects recombinant rat and human beta-arrestin and beta-arrestin2. This antibody does not detect visual or cone arrestin.
PA1-730 has been successfully used in Western blot, Immunohistochemistry (paraffin) and immunoprecipitation procedures. By Western blot, this antibody detects ~49 kDa and ~47 kDa proteins representing recombinant beta-arrestin and beta-arrestin2, respectively.
The PA1-730 immunizing peptide corresponds to amino acid residues 384-397 from human beta-arrestin2. This peptide (Cat. # PEP-156) is available for use in neutralization and control experiments.
靶标信息
Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold maolecule, beta Arrestin 1 is essential for mitogenic signalling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosporylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1&2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1&2 signaling pathway.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: arrestin 2; arrestin 3; Arrestin beta-1; Arrestin beta-2; arrestin, beta 1; arrestin, beta 2; Beta-arrestin-1; Beta-arrestin-2; Non-visual arrestin-2; Non-visual arrestin-3
基因别名: ARB1; ARB2; ARR1; ARR2; ARRB1; ARRB2; BARR2; BARRES
UniProt ID: (Human) P49407, (Human) P32121, (Rat) P29066, (Rat) P29067
Entrez Gene ID: (Human) 408, (Human) 409, (Rat) 25387, (Rat) 25388
histone acetyltransferase activity
enzyme inhibitor activity
GTPase activator activity
insulin-like growth factor receptor binding
protein binding
transcription factor binding
estrogen receptor binding
mitogen-activated protein kinase kinase binding
ubiquitin protein ligase binding
alpha-1A adrenergic receptor binding
alpha-1B adrenergic receptor binding
angiotensin receptor binding
follicle-stimulating hormone receptor binding
V2 vasopressin receptor binding
AP-2 adaptor complex binding
clathrin adaptor activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
transcription regulatory region DNA binding
ion channel binding
protein phosphorylated amino acid binding
G-protein coupled receptor binding
receptor binding
enzyme binding
protein domain specific binding
type 1 angiotensin receptor binding
D1 dopamine receptor binding
type 2A serotonin receptor binding
platelet activating factor receptor binding
protein complex binding
protein complex scaffold
protein kinase B binding
mitogen-activated protein kinase binding
14-3-3 protein binding
clathrin binding
phosphoprotein binding
scaffold/adaptor protein
activation of MAPK activity
positive regulation of protein phosphorylation
G-protein coupled receptor internalization
positive regulation of receptor internalization
transcription from RNA polymerase II promoter
apoptotic process
phototransduction
protein transport
protein ubiquitination
platelet activation
negative regulation of protein ubiquitination
positive regulation of protein ubiquitination
negative regulation of NF-kappaB transcription factor activity
positive regulation of protein binding
negative regulation of interleukin-6 production
negative regulation of interleukin-8 production
positive regulation of peptidyl-serine phosphorylation
negative regulation of GTPase activity
positive regulation of smooth muscle cell apoptotic process
positive regulation of Rho protein signal transduction
positive regulation of histone acetylation
positive regulation of insulin secretion involved in cellular response to glucose stimulus
response to antidepressant
follicle-stimulating hormone signaling pathway
stress fiber assembly
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of GTPase activity
positive regulation of transcription from RNA polymerase II promoter
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of histone H4 acetylation
desensitization of G-protein coupled receptor protein signaling pathway by arrestin
transforming growth factor beta receptor signaling pathway
dopamine receptor signaling pathway
brain development
adult walking behavior
receptor internalization
positive regulation of synaptic transmission, dopaminergic
negative regulation of interleukin-1 beta production
negative regulation of interleukin-12 production
negative regulation of tumor necrosis factor production
negative regulation of toll-like receptor signaling pathway
negative regulation of smooth muscle cell apoptotic process
negative regulation of natural killer cell mediated cytotoxicity
positive regulation of peptidyl-tyrosine phosphorylation
detection of temperature stimulus involved in sensory perception of pain
positive regulation of protein kinase B signaling
negative regulation of protein kinase B signaling
positive regulation of calcium ion transport
Wnt signaling pathway, planar cell polarity pathway
cell chemotaxis
regulation of androgen receptor signaling pathway
negative regulation of release of cytochrome c from mitochondria
chemical synaptic transmission, postsynaptic
positive regulation of DNA biosynthetic process
negative regulation of protein phosphorylation
endocytosis
receptor-mediated endocytosis
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
regulation of G-protein coupled receptor protein signaling pathway
sensory perception of pain
regulation of cAMP biosynthetic process
regulation of cAMP catabolic process
sensory perception of touch
regulation of protein phosphorylation
