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c-Abl Antibody (MA5-14398) in ICC/IF
Immunofluorescent analysis of c-Abl (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a c-Abl monoclonal antibody (Product # MA5-14398) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conju... View More
产品信息
MA5-14398
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, lambda
分类
Monoclonal
类型
Antibody
克隆号
8E9
抗原
Recombinant Abl protein
偶联物
形式
Liquid
浓度
0.4 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10983485
产品详细信息
MA5-14398 targets c-Abl in immunofluorescence, immunoprecipitation, immunohistochemistry (paraffin), and Western blot applications and shows reactivity with Human.
The MA5-14398 immunogen is recombinant Abl protein.
靶标信息
The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns c-Abl into an oncogene. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for c-Abl. In chronic myelogenous leukemia and a subset of acute lymphoblastic leukemias, the c-Abl proto oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome). As the result of the fusion of c-Abl sequences from chromosome 9 to the Bcr gene on chromosome 22. The c-Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). c-ABL potentially regulates DNA repair by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Abl 1 antib; ABL2; ABLL; ARG; B30; bcr/c-abl oncogene protein; c-abl oncogene 1, receptor tyrosine kinase; p150; Proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; RP11-83J21.1; Tyrosine kinase ARG; Tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene homolog 1
基因别名: ABL; ABL1; bcr/abl; c-ABL; c-ABL1; JTK7; p150; v-abl
UniProt ID: (Human) P00519
Entrez Gene ID: (Human) 25
magnesium ion binding
DNA binding
actin monomer binding
nicotinate-nucleotide adenylyltransferase activity
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein kinase C binding
receptor binding
protein binding
ATP binding
protein C-terminus binding
SH3 domain binding
syntaxin binding
manganese ion binding
actin filament binding
mitogen-activated protein kinase binding
proline-rich region binding
B-1 B cell homeostasis
positive regulation of protein phosphorylation
B cell proliferation involved in immune response
transitional one stage B cell differentiation
mismatch repair
regulation of transcription, DNA-templated
cellular protein modification process
autophagy
cellular response to DNA damage stimulus
DNA damage induced protein phosphorylation
response to oxidative stress
cell cycle arrest
mitotic nuclear division
epidermal growth factor receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
intrinsic apoptotic signaling pathway in response to DNA damage
regulation of autophagy
peptidyl-tyrosine phosphorylation
cerebellum morphogenesis
negative regulation of cell-cell adhesion
microspike assembly
actin cytoskeleton organization
regulation of endocytosis
regulation of cell adhesion
negative regulation of BMP signaling pathway
regulation of axon extension
regulation of microtubule polymerization
regulation of actin cytoskeleton organization
positive regulation of osteoblast proliferation
substrate adhesion-dependent cell spreading
cellular response to oxidative stress
platelet-derived growth factor receptor-beta signaling pathway
peptidyl-tyrosine autophosphorylation
Fc-gamma receptor signaling pathway involved in phagocytosis
regulation of cell proliferation
signal transduction in response to DNA damage
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
establishment of protein localization
negative regulation of mitotic cell cycle
positive regulation of mitotic cell cycle
alpha-beta T cell differentiation
protein autophosphorylation
spleen development
thymus development
collateral sprouting
positive regulation of peptidyl-tyrosine phosphorylation
activated T cell proliferation
B cell receptor signaling pathway
neuromuscular process controlling balance
positive regulation of muscle cell differentiation
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of oxidoreductase activity
negative regulation of ubiquitin-protein transferase activity
mitochondrial depolarization
Bergmann glial cell differentiation
cellular response to hydrogen peroxide
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
negative regulation of protein serine/threonine kinase activity
actin filament branching
positive regulation of interleukin-2 secretion
negative regulation of phospholipase C activity
positive regulation of neuron death
positive regulation of interferon-gamma secretion
regulation of extracellular matrix organization
cellular response to dopamine
positive regulation of microtubule binding
positive regulation of actin filament binding
activation of protein kinase C activity
positive regulation of Wnt signaling pathway, planar cell polarity pathway
regulation of cell motility
regulation of actin cytoskeleton reorganization
negative regulation of endothelial cell apoptotic process
negative regulation of cellular senescence
regulation of response to DNA damage stimulus
