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Invitrogen

c-Fos Monoclonal Antibody (T.142.5)

高级验证

3个已发表图片

9 篇参考文献

查看其他54个c-Fos抗体

说明书

实验方案

技术支持

货号 MA5-15055

CNY 5,153.00

100 µL

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说明书

实验方案

技术支持

Application

c-Fos Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

c-Fos Antibody (MA5-15055) in ICC/IF

Immunofluorescence analysis of c-Fos was performed using 70% confluent log phase HeLa cells treated with 200 ng/mL EGF for 30 min. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with c-Fos Monoclonal Antibody (T.142.5) (product # MA5-15055) at 1:250 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antib... View More

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c-Fos Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of c-Fos using c-Fos Monoclonal Antibody (T.142.5) (Product # MA5-15055) shows increased expression of c-Fos in A-431 and NIH/3T3 cell lines upon EGF and Serum Starvation followed by Serum Release treatments. {TM}

产品信息

MA5-15055

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:3,200-1:12,800

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1:1,600-1:6,400

染色质免疫沉淀实验 (ChIP)

1:50

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Bovine, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

T.142.5

抗原

Synthetic peptide corresponding to the sequence of human c-Fos

偶联物

Unconjugated

形式

Liquid

浓度

108 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10984728

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other Fos proteins, including FosB, FRA1 and FRA2.

靶标信息

FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Pharmacological inhibition of RAS overcomes FLT3 inhibitor resistance in FLT3-ITD+ AML through AP-1 and RUNX1.

iScience

Coleman DJL,Keane P,Chin PS,Ames L,Kellaway S,Blair H,Khan N,Griffin J,Holmes E,Maytum A,Potluri S,Strate L,Koscielniak K,Raghavan M,Bushweller J,Heidenreich O,Rabbitts T,Cockerill PN,Bonifer C

Published figure using c-Fos monoclonal antibody (Product # MA5-15055) in ChIP Assay

Fri Apr 19 00:00:00 UTC 2024

Leukemic stem cells activate lineage inappropriate signalling pathways to promote their growth.

Nature communications

Kellaway SG,Potluri S,Keane P,Blair HJ,Ames L,Worker A,Chin PS,Ptasinska A,Derevyanko PK,Adamo A,Coleman DJL,Khan N,Assi SA,Krippner-Heidenreich A,Raghavan M,Cockerill PN,Heidenreich O,Bonifer C

Published figure using c-Fos monoclonal antibody (Product # MA5-15055) in ChIP Assay

Wed Feb 14 00:00:00 UTC 2024

Glucose-dependent insulinotropic polypeptide regulates body weight and food intake via GABAergic neurons in mice.

Nature metabolism

Liskiewicz A,Khalil A,Liskiewicz D,Novikoff A,Grandl G,Maity-Kumar G,Gutgesell RM,Bakhti M,Bastidas-Ponce A,Czarnecki O,Makris K,Lickert H,Feuchtinger A,Tost M,Coupland C,Ständer L,Akindehin S,Prakash S,Abrar F,Castelino RL,He Y,Knerr PJ,Yang B,Hogendorf WFJ,Zhang S,Hofmann SM,Finan B,DiMarchi RD,Tschöp MH,Douros JD,Müller TD

MA5-15055 was used in Immunohistochemistry (Frozen) to demonstrate that long-acting GIPR agonists crucially depend on GIPR signaling in inhibitory GABAergic neurons to decrease body weight and food intake.

Fri Dec 01 00:00:00 UTC 2023

Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.

Frontiers in nutrition

Long X,Wu H,Zhou Y,Wan Y,Kan X,Gong J,Zhao X

MA5-15055 was used in Western Blotting to show that L. fermentum SCHY34 showed a great biological scavenging and potential effect on alleviating the toxicity of lead ions.

Sat Jul 16 00:00:00 UTC 2022

TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling.

eLife

Tang Y,Zong H,Kwon H,Qiu Y,Pessin JB,Wu L,Buddo KA,Boykov I,Schmidt CA,Lin CT,Neufer PD,Schwartz GJ,Kurland IJ,Pessin JE

MA5-15055 was used in Western Blot to demonstrate that increased cholinergic signaling through the modulation of glycolysis has several metabolic benefits particularly to increase energy expenditure and heat production upon cold exposure.

Mon Mar 07 00:00:00 UTC 2022

Bidirectional regulation between AP-1 and SUMOylation pathway genes modulates inflammatory signaling during Salmonella infection.

Journal of cell science

Kumar P,Soory A,Mustfa SA,Sarmah DT,Devvanshi H,Chatterjee S,Bossis G,Ratnaparkhi GS,Srikanth CV

MA5-15055 was used in Immunocytochemistry to investigate the regulation of SUMO pathway genes during Salmonella Typhimurium (STm) infection.

Mon Aug 15 00:00:00 UTC 2022

Functional responses of the hippocampus to hyperexcitability depend on directed, neuron-specific KCNQ2 K⁺ channel plasticity.

Hippocampus

Carver CM,Hastings SD,Cook ME,Shapiro MS

MA5-15055 was used in Immunohistochemistry to demonstrate that increased excitatory activity promotes KCNQ2 upregulation in the hippocampus in a cell-type specific manner.

Fri May 01 00:00:00 UTC 2020

Altered chromatin landscape and enhancer engagement underlie transcriptional dysregulation in MED12 mutant uterine leiomyomas.

Nature communications

Moyo MB,Parker JB,Chakravarti D

MA5-15055 was used in Western Blot to establish AP-1 driven aberrant enhancer regulation as an important mechanism of leiomyoma disease pathogenesis.

Mon Feb 24 00:00:00 UTC 2020

Environment and Behavior: Neurochemical Effects of Different Diets in the Calf Brain.

Animals : an open access journal from MDPI

Peli A,Grandis A,Tassinari M,Famigli Bergamini P,Tagliavia C,Roccaro M,Bombardi C

MA5-15055 was used in Immunohistochemistry to support the importance of maintaining a nutritional lifestyle similar to that occurring in natural conditions.

Fri Jun 14 00:00:00 UTC 2019

生物信息学

蛋白别名: activator protein 1; c-fos oncogene; cellular oncogene c-fos; Cellular oncogene fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FBJ osteosarcoma oncogene; Fos proto-oncogene, AP-1 trancription factor subunit; Fos proto-oncogene, AP-1 transcription factor subunit; G0/G1 switch regulatory protein 7; Protein c-Fos; Proto-oncogene c-Fos; proto-oncogene cFOS; proto-oncogene protein c-fos; Transcription factor AP-1 subunit c-Fos; v-fos FBJ murine osteosarcoma viral oncogene homolog

基因别名: AP-1; C-FOS; cFos; D12Rfj1; FOS; G0S7; p55

UniProt ID: (Human) P01100, (Mouse) P01101, (Rat) P12841

Entrez Gene ID: (Human) 2353, (Mouse) 14281, (Rat) 314322

功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding transcription regulatory region DNA binding protein heterodimerization activity R-SMAD binding DNA binding double-stranded DNA binding sequence-specific DNA binding basic leucine zipper transcription factor

参与通路

conditioned taste aversion DNA methylation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter inflammatory response transforming growth factor beta receptor signaling pathway nervous system development female pregnancy aging response to cold response to light stimulus response to gravity response to toxic substance sleep cellular response to extracellular stimulus response to lipopolysaccharide response to progesterone cellular response to hormone stimulus response to cytokine cellular response to reactive oxygen species response to immobilization stress skeletal muscle cell differentiation response to muscle stretch Fc-epsilon receptor signaling pathway response to drug positive regulation of osteoclast differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of sequence-specific DNA binding transcription factor activity response to corticosterone response to cAMP SMAD protein signal transduction cellular response to calcium ion positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of transcription, DNA-templated response to mechanical stimulus response to organic cyclic compound

c-Fos Monoclonal Antibody (T.142.5)

c-Fos Antibody (MA5-15055) in ICC/IF

产品信息

MA5-15055

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

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c-Fos Monoclonal Antibody (T.142.5)

c-Fos Antibody (MA5-15055) in ICC/IF

产品信息

MA5-15055

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)

RRID