Bethyl Laboratories

c-Jun Polyclonal Antibody

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抗体检测数据 (2)

c-Jun Antibody in Immunohistochemistry (IHC)

c-Jun Antibody in Immunohistochemistry (IHC)

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c-Jun Antibody (A302-958A) in IHC

Detection of human c-Jun by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- c-Jun (Cat. No. A302-958A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.

c-Jun Antibody in Immunohistochemistry (IHC)

c-Jun Antibody in Western Blot (WB)

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c-Jun Antibody in Immunohistochemistry (IHC) — Detection of human c-Jun by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- c-Jun (Cat. No. A302-958A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.

c-Jun Antibody in Western Blot (WB) — Detection of human and mouse c-Jun by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from 293T (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-c-Jun antibody A302-958A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. c-Jun was also immunoprecipitated by rabbit anti-c-Jun antibody A302-959A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

产品信息

A302-958A

应用

建议稀释比

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免疫印迹 (WB)

1:2,000-1:10,000

-

免疫组化 (IHC)

1:100-1:500

-

免疫沉淀 (IP)

2-5 µg/mg lysate

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 1 and 40 of human Jun Oncogene.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.20 mg/mL

纯化类型

Antigen affinity chromatography

保存液

TBS, pH 7.0 to 8.0, with 0.1% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

靶标信息

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Activator protein 1; AH119; AP1; cjun; enhancer-binding protein AP1; immediate early; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; JUND; p39; Proto-oncogene c-Jun; transcription factor AP-1; Transcription factor AP-1 subunit Jun; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog

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基因别名: AP-1; AP1; c-Jun; JUN; Junc

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UniProt ID: (Human) P05412, (Mouse) P05627

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Entrez Gene ID: (Human) 3725, (Mouse) 16476

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RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity GTPase activator activity protein binding transcription factor binding enzyme binding cAMP response element binding identical protein binding protein homodimerization activity transcription regulatory region DNA binding poly(A) RNA binding protein heterodimerization activity R-SMAD binding HMG box domain binding double-stranded DNA binding sequence-specific DNA binding basic leucine zipper transcription factor

参与通路

angiogenesis microglial cell activation release of cytochrome c from mitochondria liver development positive regulation of endothelial cell proliferation outflow tract morphogenesis transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway SMAD protein import into nucleus Ras protein signal transduction aging learning circadian rhythm negative regulation of cell proliferation response to radiation response to mechanical stimulus positive regulation of epithelial cell migration regulation of cell death monocyte differentiation axon regeneration negative regulation of protein autophosphorylation response to lipopolysaccharide cellular response to hormone stimulus response to cytokine leading edge cell differentiation response to muscle stretch Fc-epsilon receptor signaling pathway regulation of cell proliferation response to drug response to hydrogen peroxide negative regulation of DNA binding negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process positive regulation of GTPase activity negative regulation by host of viral transcription positive regulation by host of viral transcription positive regulation of cell differentiation positive regulation of monocyte differentiation positive regulation of DNA replication negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation regulation of sequence-specific DNA binding transcription factor activity cellular response to potassium ion starvation response to cAMP regulation of cell cycle membrane depolarization SMAD protein signal transduction eyelid development in camera-type eye positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress positive regulation of DNA-templated transcription, initiation negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of cell proliferation cellular process positive regulation of apoptotic process negative regulation of apoptotic process