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Invitrogen
c-Jun Recombinant Rabbit Monoclonal Antibody (2H4L1)
高级验证
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
图: 1 / 7
c-Jun Antibody (702170) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized HeLa cells for detection of endogenous c-jun using Anti-c-jun Recombinant Rabbit Monoclonal Antibody (Product # 702170, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of c-jun protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938)... View More
产品信息
702170
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
2H4L1
抗原
Peptides corresponding to Human JUN (aa 21-36, 211-228)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633062
产品详细信息
This antibody is predicted to react with Monkey, Bovine and Pig
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Activator protein 1; AH119; AP1; cjun; enhancer-binding protein AP1; immediate early; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; JUND; p39; Proto-oncogene c-Jun; transcription factor AP-1; Transcription factor AP-1 subunit Jun; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog
基因别名: AP-1; AP1; c-Jun; JUN; Junc
UniProt ID: (Human) P05412, (Mouse) P05627
Entrez Gene ID: (Human) 3725, (Mouse) 16476
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
transcriptional activator activity, RNA polymerase II transcription factor binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
GTPase activator activity
protein binding
transcription factor binding
enzyme binding
cAMP response element binding
identical protein binding
protein homodimerization activity
transcription regulatory region DNA binding
poly(A) RNA binding
protein heterodimerization activity
R-SMAD binding
HMG box domain binding
double-stranded DNA binding
sequence-specific DNA binding
basic leucine zipper transcription factor
angiogenesis
microglial cell activation
release of cytochrome c from mitochondria
liver development
positive regulation of endothelial cell proliferation
outflow tract morphogenesis
transcription from RNA polymerase II promoter
transforming growth factor beta receptor signaling pathway
SMAD protein import into nucleus
Ras protein signal transduction
aging
learning
circadian rhythm
negative regulation of cell proliferation
response to radiation
response to mechanical stimulus
positive regulation of epithelial cell migration
regulation of cell death
monocyte differentiation
axon regeneration
negative regulation of protein autophosphorylation
response to lipopolysaccharide
cellular response to hormone stimulus
response to cytokine
leading edge cell differentiation
response to muscle stretch
Fc-epsilon receptor signaling pathway
regulation of cell proliferation
response to drug
response to hydrogen peroxide
negative regulation of DNA binding
negative regulation of neuron apoptotic process
positive regulation of neuron apoptotic process
positive regulation of GTPase activity
negative regulation by host of viral transcription
positive regulation by host of viral transcription
positive regulation of cell differentiation
positive regulation of monocyte differentiation
positive regulation of DNA replication
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
positive regulation of smooth muscle cell proliferation
regulation of sequence-specific DNA binding transcription factor activity
cellular response to potassium ion starvation
response to cAMP
regulation of cell cycle
membrane depolarization
SMAD protein signal transduction
eyelid development in camera-type eye
positive regulation of ERK1 and ERK2 cascade
cellular response to calcium ion
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
positive regulation of DNA-templated transcription, initiation
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of cell proliferation
cellular process
positive regulation of apoptotic process
negative regulation of apoptotic process
