Invitrogen

c-Jun Recombinant Rabbit Monoclonal Antibody (SY0298)

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

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抗体检测数据 (6)
高级验证 (1)

c-Jun Antibody in Immunocytochemistry (ICC/IF)

c-Jun Antibody in Immunocytochemistry (ICC/IF)

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c-Jun Antibody (MA5-41123) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of c-Jun in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with c-Jun Recombinant Monoclonal Antibody (Product # MA5-41123) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

c-Jun Antibody in Immunocytochemistry (ICC/IF)

c-Jun Antibody in Immunocytochemistry (ICC/IF)

c-Jun Antibody in Immunocytochemistry (ICC/IF)

c-Jun Antibody in Immunohistochemistry (Paraffin) (IHC (P))

c-Jun Antibody in Western Blot (WB)

c-Jun Antibody in Western Blot (WB)

Product Image Gallery

c-Jun Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis of c-Jun in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with c-Jun Recombinant Monoclonal Antibody (Product # MA5-41123) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

c-Jun Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis of c-Jun in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with c-Jun Recombinant Monoclonal Antibody (Product # MA5-41123) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

c-Jun Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis of c-Jun in L6 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with c-Jun Recombinant Monoclonal Antibody (Product # MA5-41123) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

c-Jun Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of c-Jun in paraffin-embedded human breast carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with c-Jun Recombinant Monoclonal Antibody (Product # MA5-41123) at a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

c-Jun Antibody in Western Blot (WB) — Western Blot analysis of c-Jun using c-Jun Recombinant Monoclonal Antibody (Product # MA5-41123) at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: Hela cell lysate, Lane 2: Human skin tissue lysate.

c-Jun Antibody in Western Blot (WB) — Knockout of c-JUN was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR905187_SGM) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of c-JUN was performed by loading 30 µg of HEK-293 wild type (Lane 1), HEK-293 Cas9 (Lane 2) and HEK-293 c-JUN KO (Lane 3) modified whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with c-Jun Recombinant Rabbit Monoclonal Antibody (SY0298) (Product # MA5-41123, 1:1000 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution) using the iBright™ FL1500 (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to c-JUN. An uncharacterized band was observed in HEK-293 WT sample t ~25 kDa.

c-Jun Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inc-JUN KO cell line compared to control cell line using c-Jun Recombinant Rabbit Monoclonal Antibody (SY0298) (Product # MA5-41123). {KO}

产品信息

MA5-41123

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SY0298

抗原

Synthetic peptide within N-terminal human c-Jun

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2898877

靶标信息

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Activator protein 1; AH119; AP1; Avian sarcoma virus 17 (v-jun) oncogene homolog; cjun; enhancer-binding protein AP1; immediate early; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; JUND; p39; Proto-oncogene c-Jun; transcription factor AP-1; Transcription factor AP-1 subunit Jun; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog

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基因别名: AP-1; AP1; c-Jun; JUN; Junc; Rjg-9

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UniProt ID: (Human) P05412, (Rat) P17325, (Mouse) P05627

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Entrez Gene ID: (Human) 3725, (Rat) 24516, (Mouse) 16476

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RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity GTPase activator activity protein binding transcription factor binding enzyme binding cAMP response element binding identical protein binding protein homodimerization activity transcription regulatory region DNA binding poly(A) RNA binding protein heterodimerization activity R-SMAD binding HMG box domain binding double-stranded DNA binding sequence-specific DNA binding basic leucine zipper transcription factor

参与通路

angiogenesis microglial cell activation release of cytochrome c from mitochondria liver development positive regulation of endothelial cell proliferation outflow tract morphogenesis transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway SMAD protein import into nucleus Ras protein signal transduction aging learning circadian rhythm negative regulation of cell proliferation response to radiation response to mechanical stimulus positive regulation of epithelial cell migration regulation of cell death monocyte differentiation axon regeneration negative regulation of protein autophosphorylation response to lipopolysaccharide cellular response to hormone stimulus response to cytokine leading edge cell differentiation response to muscle stretch Fc-epsilon receptor signaling pathway regulation of cell proliferation response to drug response to hydrogen peroxide negative regulation of DNA binding negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process positive regulation of GTPase activity negative regulation by host of viral transcription positive regulation by host of viral transcription positive regulation of cell differentiation positive regulation of monocyte differentiation positive regulation of DNA replication negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation regulation of sequence-specific DNA binding transcription factor activity cellular response to potassium ion starvation response to cAMP regulation of cell cycle membrane depolarization SMAD protein signal transduction eyelid development in camera-type eye positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress positive regulation of DNA-templated transcription, initiation transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of cell proliferation cellular process response to organic substance response to organic cyclic compound positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of transcription from RNA polymerase II promoter