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Invitrogen

eIF3i Polyclonal Antibody

高级验证

查看其他7个eIF3i抗体

说明书

实验方案

问题与答案

技术支持

货号 PA5-56302

CNY 4,595.00

100 µL

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说明书

实验方案

问题与答案

技术支持

抗体检测数据 (7)
高级验证 (1)

Application

eIF3i Antibody in Immunohistochemistry (IHC)

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eIF3i Antibody (PA5-56302) in IHC

Immunohistochemical staining of eIF3i in human cerebral cortex, lymph node, rectum and testis using eIF3i Polyclonal Antibody (Product # PA5-56302) (A) shows similar protein distribution across tissues to an independent eIF3i Polyclonal Antibody (B).

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eIF3i Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-251 mg cells were transfected with, control siRNA, eIF3i siRNA probe #1, and eIF3i siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-eIF3i Polyclonal Antibody (Product # PA5-56302) used at a dilution of 0.4 µg/mL. Anti-PPIB was used as a loading control. {KD}

产品信息

PA5-56302

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human eIF3i. Recombinant protein control fragment (Product #RP-94035).

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偶联物

Unconjugated

形式

Liquid

浓度

0.05 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2640967

产品详细信息

Immunogen sequence: KYNREGDLLF TVAKDPIVNV WYSVNGERLG TYMGHTGAVW CVDADWDTKH VLTGSADNSC RLWDCETGKQ LALLKTNSAV RTCG

Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 87%.

靶标信息

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: eIF-3-beta; eIF3 p36; eIF3i; Eukaryotic translation initiation factor 3 subunit 2; Eukaryotic translation initiation factor 3 subunit I; eukaryotic translation initiation factor 3, subunit 1; eukaryotic translation initiation factor 3, subunit 2 (beta); eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD); eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa; eukaryotic translation initiation factor 3, subunit I; predicted protein of HQ2242; subuni; TGF-beta receptor binding protein; TGF-beta receptor-interacting protein 1; TGFbeta receptor-interacting protein 1; TRIP-1

基因别名: 36kDa; D4Ertd632e; eIF3-beta; eIF3-p36; EIF3I; EIF3S2; PRO2242; TRIP-1; TRIP1

UniProt ID: (Human) Q13347, (Mouse) Q9QZD9, (Rat) B0BNA7

Entrez Gene ID: (Human) 8668, (Mouse) 54709, (Rat) 682390