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Invitrogen
eIF4A1 Polyclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 6
eIF4A1 Antibody (PA5-30216) in ICC/IF
Immunofluorescence analysis of eIF4A1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with eIF4A1 Polyclonal Antibody (Product # PA5-30216) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugat... View More
产品信息
PA5-30216
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 69 and 284 of Human EIF4A1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2547690
产品详细信息
Recommended positive controls: HeLa.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (95%), Dog (100%), Pig (100%), Chimpanzee (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amounts.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATP-dependent RNA helicase eIF4A-1; eIF-4A-I; eIF4A-I; Eukaryotic initiation factor 4A-I; eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1; initiation factor eIF-4A long form
基因别名: BM-010; DDX2A; EIF-4A; eIF-4A-I; Eif4; EIF4A; eIF4A-I; EIF4A1
UniProt ID: (Human) P60842, (Mouse) P60843
Entrez Gene ID: (Human) 1973, (Mouse) 13681, (Rat) 287436
RNA cap binding
double-stranded RNA binding
mRNA binding
translation initiation factor activity
ATP-dependent RNA helicase activity
helicase activity
protein binding
ATP binding
translation factor activity, RNA binding
poly(A) RNA binding
nucleotide binding
nucleic acid binding
RNA binding
hydrolase activity
molecular_function
