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hnRNP D Antibody (PA5-75200) in ICC/IF
Immunofluorescence analysis of hnRNP D was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with hnRNP D Polyclonal Antibody (Product # PA5-75200) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product ... View More
产品信息
PA5-75200
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to amino acids 46-98 of Human hnRNP D.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2718928
产品详细信息
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
靶标信息
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ARE-binding protein AUFI, type A; AU-rich element RNA binding protein 1, 37kDa; AU-rich element RNA-binding factor 1; AU-rich element RNA-binding protein 1; heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa); Heterogeneous nuclear ribonucleoprotein D0; hnRNP D0; RNA binding protein p45AUF1
基因别名: AUF1; AUF1A; HNRNPD; hnRNPD0; HNRPD; P37
UniProt ID: (Human) Q14103, (Mouse) Q60668, (Rat) Q9JJ54
Entrez Gene ID: (Human) 3184, (Mouse) 11991, (Rat) 79256
nuclear-transcribed mRNA catabolic process, exonucleolytic
nucleocytoplasmic transport
regulation of circadian rhythm
regulation of mRNA stability
positive regulation of translation
mRNA stabilization
circadian regulation of translation
mRNA splicing, via spliceosome
liver development
transcription, DNA-templated
regulation of transcription, DNA-templated
RNA processing
RNA catabolic process
gene expression
response to fluoxetine
cerebellum development
positive regulation of transcription, DNA-templated
response to calcium ion
response to electrical stimulus
3'-UTR-mediated mRNA destabilization
cellular response to amino acid stimulus
cellular response to estradiol stimulus
cellular response to nitric oxide
response to rapamycin
response to sodium phosphate
cellular response to putrescine
hepatocyte dedifferentiation
rhythmic process
