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mCherry Antibody (740003TP555) in WB
Western blot was performed using mCherry Recombinant Rat Monoclonal Antibody (16D7), Alexa Fluor™ Plus 555 (Product # 740003TP555) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: Untransfected (10 µg, Lane 1), empty vector control (10 µg, Lane 2), H3-mCherry (10 µg, Lane 3), H3-mCherry (5 µg, Lane 4), mCherry-GST (1 µg, Lane 5), and GST-mCherry (1 µg, Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitro... View More
产品信息
740003TP555
产品规格
种属反应
Tag
宿主/亚型
Rat
/ IgG2a, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
16D7
抗原
Full-length protein mCherry
偶联物
激发/发射光谱
558/572 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein G
保存液
proprietary buffer, pH 6.8
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
mCherry Tag recombinant rat monoclonal antibody specifically recognizes mCherry Tag at both C and N-terminus of proteins.
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This 16D7 antibody has been tested by western blot and flow cytometric analysis using HEK-293E cells that were transiently transfected with mCherry-protein constructs. This may be used for western blot at 1 µg/mL and for flow cytometry at 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: mCherry
