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Invitrogen
p16INK4a Recombinant Rabbit Monoclonal Antibody (10H23L14)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 10
p16INK4a Antibody (703831) in ICC/IF
Knockdown of p16INK4a was achieved by transfecting HeLa cells with specific validated siRNA (Silencer® select Product # s218). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a-d), transfected with p16INK4a specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-p16INK4a Recombinant Rabbit Monoclonal Antibody (Product # 703831, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plu... View More
产品信息
703831
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
10H23L14
抗原
Peptides corresponding to human p16INK4a [1) aa1-aa15 2) aa137-aa156]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2848244
产品详细信息
This antibody is predicted to react with Mouse, Rat, Bovine.
靶标信息
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Alternative reading frame; ARF; CDK4 inhibitor p16 INK4; CDK4 inhibitor p16-INK4; CDK4I; cell cycle negative regulator beta; Cyclin dependent kinase 4 inhibitor A; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); HGNC:1787; inhibits CDK4; isoforms 1/2/3; Melanoma p16 inhibits CDK4; MTS-1; Multiple tumor suppressor 1; P14 P16; p14ARF; p16-INK4; p16-INK4a; P19 TP16; Tumor suppressor ARF
基因别名: ARF; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16
UniProt ID: (Human) Q8N726
Entrez Gene ID: (Human) 1029
G1/S transition of mitotic cell cycle
negative regulation of cell-matrix adhesion
cell cycle arrest
Ras protein signal transduction
negative regulation of cell proliferation
negative regulation of cell growth
negative regulation of NF-kappaB transcription factor activity
positive regulation of smooth muscle cell apoptotic process
senescence-associated heterochromatin focus assembly
negative regulation of phosphorylation
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of transcription, DNA-templated
cellular senescence
replicative senescence
positive regulation of macrophage apoptotic process
positive regulation of cellular senescence
