Invitrogen

p27 Kip1 Polyclonal Antibody

高级验证

IP-mass spectrometry

1个已发表图片

6 篇参考文献

查看其他67个p27Kip1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

p27 Kip1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

p27 Kip1 Antibody (PA5-27188) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of p27 Kip1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: p27 Kip1 Polyclonal Antibody (Product # PA5 27188) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

p27 Kip1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

p27 Kip1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

p27 Kip1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

PA5-27188

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 55 and 198 of Human P27 Kip1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.08 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2544664

产品详细信息

Recommended positive controls: HeLa, MCF-7, MDA-MB-231, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12.

Predicted reactivity: Mouse (80%), Dog (87%), Cat (87%), Pig (84%), Rhesus Monkey (98%), Bovine (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Bacterial protein MakA causes suppression of tumour cell proliferation via inhibition of PIP5K1α/Akt signalling.

Cell death & disease

Toh E,Baryalai P,Nadeem A,Aung KM,Chen S,Persson K,Persson JL,Uhlin BE,Wai SN

PA5-27188 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to indicate that MakA could affect eukaryotic cell signalling pathways involved in lipid biosynthesis.

Tue Dec 06 00:00:00 EST 2022

Senolysis-Based Elimination of Chemotherapy-Induced Senescent Breast Cancer Cells by Quercetin Derivative with Blocked Hydroxy Groups.

Cancers

Lewińska A,Przybylski P,Adamczyk-Grochala J,Błoniarz D,Litwinienko G,Wnuk M

PA5-27188 was used in Immunocytochemistry-immunofluorescence to study targeted elimination of senescent cells using synthesized quercetin derivatives which were tested for improved senolytic action against etoposide-induced senescent human normal mammary epithelial cells and triple-negative breast cancer cells in vitro.

Tue Jan 25 00:00:00 EST 2022

Human 3D cellular model of hypoxic brain injury of prematurity.

Nature medicine

Pașca AM,Park JY,Shin HW,Qi Q,Revah O,Krasnoff R,O'Hara R,Willsey AJ,Palmer TD,Pașca SP

PA5-27188 was used in Immunocytochemistry-immunoflourescence to anticipate that this human cellular platform will be valuable for studying the environmental and genetic factors underlying injury in the developing human brain.

Wed May 01 00:00:00 EDT 2019

CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine.

PLoS biology

Ale-Agha N,Goy C,Jakobs P,Spyridopoulos I,Gonnissen S,Dyballa-Rukes N,Aufenvenne K,von Ameln F,Zurek M,Spannbrucker T,Eckermann O,Jakob S,Gorressen S,Abrams M,Grandoch M,Fischer JW,Köhrer K,Deenen R,Unfried K,Altschmied J,Haendeler J

PA5-27188 was used in Immunohistochemistry-immunofluorescence to show that the cyclin-dependent kinase inhibitor 1B (CDKN1B)/p27, previously known as a cell cycle inhibitor, is also localized within mitochondria.

Fri Jun 01 00:00:00 EDT 2018

ESRP1 Mutations Cause Hearing Loss due to Defects in Alternative Splicing that Disrupt Cochlear Development.

Developmental cell

Rohacek AM,Bebee TW,Tilton RK,Radens CM,McDermott-Roe C,Peart N,Kaur M,Zaykaner M,Cieply B,Musunuru K,Barash Y,Germiller JA,Krantz ID,Carstens RP,Epstein DJ

PA5-27188 was used in Immunohistochemistry to elucidate the importance of mutations in ESRP1 as a cause of sensorineural hearing loss, and demonstrate the complex interplay between alternative splicing, inner ear development, and auditory function.

Mon Nov 06 00:00:00 EST 2017

Context-Dependent Functional Divergence of the Notch Ligands DLL1 and DLL4 In Vivo.

PLoS genetics

Preuße K,Tveriakhina L,Schuster-Gossler K,Gaspar C,Rosa AI,Henrique D,Gossler A,Stauber M

PA5-27188 was used in immunohistochemistry to determine tissue specific functions of DLL1 or DLL4 using transgenic mice.

Mon Jun 01 00:00:00 EDT 2015

生物信息学

蛋白别名: CDN1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; P27 CDKN1B; p27(KIP1); p27Kip1

查看更多收起

基因别名: AA408329; AI843786; CDKN1B; CDKN4; KIP1; MEN1B; MEN4; p27; P27KIP1

查看更多收起

UniProt ID: (Human) P46527, (Mouse) P46414

查看更多收起

Entrez Gene ID: (Human) 1027, (Mouse) 12576

查看更多收起

功能

cyclin-dependent protein serine/threonine kinase inhibitor activity transforming growth factor beta receptor, cytoplasmic mediator activity protein binding cysteine-type endopeptidase activator activity involved in apoptotic process protein phosphatase binding Hsp70 protein binding protein complex binding chaperone binding protein kinase inhibitor activity kinase inhibitor

参与通路