Target of Interest
Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
图: 1 / 3
rRNA Antibody (MA1-13017) in ICC/IF
Immunofluorescence analysis of Ribosomal RNA was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ribosomal RNA 5.8S (Y10B) Mouse Monoclonal Antibody (Product # MA1-13017) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj... View More
产品信息
MA1-13017
应用
建议稀释比
已发表文章
产品规格
种属反应
Many
已发表种属
Fruit fly,
Mouse,
Zebrafish
宿主/亚型
Mouse
/ IgG3, kappa
分类
Monoclonal
类型
Antibody
克隆号
Y10b
抗原
Whole ribosomal rRNA 5.8S
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10979967
产品详细信息
This clone recognizes both the large and the small subunit of rRNA from all eukaryotic cells.
MA1-13017 has been successfully used in Immunocytochemistry, immunoprecipitation, immunofluorescence applications.
The MA1-13017 immunogen is from whole ribosomal rRNA 52.8S
靶标信息
rRNA (ribosomal RNA) forms a major structural component of ribosomes which provides a mechanism for decoding mRNA information into amino acids/proteins through the process of translation. rRNA plays a key role in accurate positioning of mRNA as well as peptidyl tRNA, and also plays a structural role in peptidyl transferase activity by generating scaffolds where ribosomal proteins are assembled. rRNA is generated inside nucleolus, which is localized in nucleus of the cells and contains genes essential for encoding rRNA. Defective rRNA may implicate in certain pathologies, for example: decreased rRNA expression has been observed in subjects with myelodysplastic syndromes, and abnormal rRNA expression has also been linked to Alzheimer's disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ribosomal RNA; rRNA
