Traditional culture-based or microscopy-based methods for investigations into vaginal microbiota can lack sensitivity and specificity and can also be subjective, time-consuming and inaccurate.
Real-time PCR can detect slow-growing, difficult-to cultivate, or uncultivable microorganisms, and can be used when traditional microbiological techniques lead to ambiguous results. Using real-time PCR techniques along with microorganism-specific TaqMan Assays enables rapid and accurate detection and categorization of microorganisms involved in bacterial vaginosis, aerobic vaginitis and vaginal candidiasis.
We have developed a flexible, low-cost and high-throughput real-time PCR testing solution for vaginal microbiota profiling.* This solution includes a single protocol optimized for DNA isolation from different types of vaginal microbes, a set of validated TaqMan Assays covering 28 pathogenic and commensal microbes, plus four control assays, including human (RNase P) and bacterial (16S) targets.
Key Features:
- The ability to detect the broadest range of both commensal and pathogenic microbes compared to other commercially available molecular tests
- Qualified content, including positive controls, user guide and analytical testing
- Higher specificity, accuracy and precision compared to traditional culture and microscopy methods
- Flexible formats—choose from four assay formats, including single-tube assays and preloaded 384-well or OpenArray™ plates
- Lowest cost per sample compared to other commercially available solutions
Find out more about our vaginal microbiota PCR solutions at thermofisher.com/vm
*For research use only. Not for use in diagnostic procedures.
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