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BANF1 Antibody (PA5-20328) in WB
Western Blot Validation in Human Kidney Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: BANF1 Polyclonal Antibody (Product # PA5-20328) (A: 0.5 µg/mL and B: 1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
产品信息
PA5-20328
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A 15 amino acid peptide from near the carboxy terminus of human BANF1.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
0.02% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_11153647
产品详细信息
Despite its predicted molecular weight, BANF1 often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is human kidney tissue lysate.
PA5-20328 can be used with blocking peptide PEP-0448.
靶标信息
Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BANF1 also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BANF1 will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BANF1 is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Barrier-to-autointegration factor; Breakpoint cluster region protein 1; LAP2-binding protein 1
基因别名: BAF; BANF1; BCRG1; BCRP1; D14S1460; L2bp1; L2bp1/Baf; NGPS
UniProt ID: (Human) O75531, (Mouse) Q542E6
Entrez Gene ID: (Human) 8815, (Mouse) 23825
