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Invitrogen

CD31 Monoclonal Antibody (WM59)

Name: CD31 Monoclonal Antibody (WM59)
Brand: Invitrogen
SKU: MA126196
Price: 6233.0 CNY
Availability: InStock

高级验证

3个已发表图片

7 篇参考文献

查看其他177个CD31 (PECAM-1)抗体

说明书

实验方案

技术支持

货号 MA1-26196

飞享价 CNY 4,521.00 6,233.00

活动截止日期 31-Dec-2025

100 µg

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说明书

实验方案

技术支持

Application

CD31 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

CD31 Antibody (MA1-26196) in ICC/IF

Immunofluorescence analysis of Platelet endothelial cell adhesion molecule was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD31 Monoclonal Antibody (WM59) (Product # MA1-26196) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary An... View More

CD31 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD31 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CD31 Monoclonal Antibody (WM59) (Product # MA1-26196) was observed to be high in HUVEC in comparison to no expression in MDA-MB-231 (Ref:https://www.nature.com/articles/onc2013214). {RE}

产品信息

MA1-26196

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看6篇已发表文章 6篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫组织化学（悬浮细胞） (IHC (Free))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:50-1:100

流式细胞分析 (Flow)

1:50-1:100

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey

已发表种属

Mouse, Rabbit, Rat, Rhesus monkey

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

WM59

抗原

Human CD31

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_779679

产品详细信息

In FACS use 10 µL of the suggested working dilution to label 10^6 cels in 100 µL.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections.

靶标信息

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Endothelial cell-derived MMP19 promotes pulmonary fibrosis by inducing E(nd)MT and monocyte infiltration.

Cell communication and signaling : CCS

Zhao W,Wang L,Yang J,Chen X,Guo X,Xu K,Wang N,Zhao W,Xia C,Lian H,Rosas I,Yu G

MA1-26196 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry - Free Floating, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation to find that MMP19 expression was significantly increased in the lung endothelial cells of IPF patients and BLM-induced mice compared to the control groups.

Mon Mar 13 00:00:00 UTC 2023

An instantly fixable and self-adaptive scaffold for skull regeneration by autologous stem cell recruitment and angiogenesis.

Nature communications

Lu G,Xu Y,Liu Q,Chen M,Sun H,Wang P,Li X,Wang Y,Li X,Hui X,Luo E,Liu J,Jiang Q,Liang J,Fan Y,Sun Y,Zhang X

MA1-26196 was used in Immunohistochemistry to design an instantly fixable and self-adaptive scaffold by dopamine-modified hyaluronic acid chelating Ca2+ of the microhydroxyapatite surface and bonding type I collagen to highly simulate the natural bony matrix.

Fri May 06 00:00:00 UTC 2022

Micro-anatomic alterations of the placenta in a non-human primate model of gestational protein-restriction.

PloS one

Sargent J,Roberts V,D'Souza K,Wright A,Gaffney J,Frias A

MA1-26196 was used in Immunohistochemistry to histologically characterize the micro-anatomic alterations associated with adverse pregnancy outcomes taking an approach that permits investigation of the 3D vascular structure and surrounding histology without the requirement for 3D vascular casting or relying on 2D stereology which both have methodological limitations.

Tue Sep 15 00:00:00 UTC 2020

Clarification and confocal imaging of the nonhuman primate placental micro-anatomy.

BioTechniques

Sargent JA,Roberts V,Gaffney JE,Frias AE

Published figure using CD31 monoclonal antibody (Product # MA1-26196) in Immunohistochemistry

Fri Feb 01 00:00:00 UTC 2019

Profiling of normal and malignant breast tissue show CD44high/CD24low phenotype as a predominant stem/progenitor marker when used in combination with Ep-CAM/CD49f markers.

BMC cancer

Ghebeh H,Sleiman GM,Manogaran PS,Al-Mazrou A,Barhoush E,Al-Mohanna FH,Tulbah A,Al-Faqeeh K,Adra CN

MA1-26196 was used in flow cytometry to determine the phenotype and function of stem/progenitor cells in normal mammary epithelial cell populations and their malignant counterparts

Fri Jun 14 00:00:00 UTC 2013

Preferential accumulation within tumors and in vivo imaging by functionalized luminescent dendrimer lanthanide complexes.

Biomaterials

Alcala MA,Shade CM,Uh H,Kwan SY,Bischof M,Thompson ZP,Gogick KA,Meier AR,Strein TG,Bartlett DL,Modzelewski RA,Lee YJ,Petoud S,Brown CK

MA1-26196 was used in immunohistochemistry to study the use of luminescent dendrimer lanthanide complexes for in vivo tumor imaging

Thu Dec 01 00:00:00 UTC 2011

Luminescence targeting and imaging using a nanoscale generation 3 dendrimer in an in vivo colorectal metastatic rat model.

Nanomedicine : nanotechnology, biology, and medicine

Alcala MA,Kwan SY,Shade CM,Lang M,Uh H,Wang M,Weber SG,Bartlett DL,Petoud S,Lee YJ

MA1-26196 was used in immunohistochemistry to study the application of generation 3 polyamidoamine 4-amino-1,8-naphthalimide dendrimer containing 8 europium ions (Eu-G3P4A18N) for luminescence imaging

Wed Jun 01 00:00:00 UTC 2011

生物信息学

蛋白别名: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

基因别名: C85791; CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; Pecam; PECAM-1; PECAM1

UniProt ID: (Human) P16284, (Mouse) Q08481

Entrez Gene ID: (Rhesus monkey) 718302, (Cynomolgus monkey) 102117792, (Human) 5175, (Mouse) 18613

功能

protein binding immunoglobulin receptor superfamily

参与通路

platelet degranulation phagocytosis cell adhesion signal transduction cell recognition extracellular matrix organization leukocyte migration diapedesis glomerular endothelium development

CD31 Monoclonal Antibody (WM59)

CD31 Antibody (MA1-26196) in ICC/IF

产品信息

MA1-26196

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

免疫组织化学（悬浮细胞） (IHC (Free))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

CD31 Monoclonal Antibody (WM59)

CD31 Antibody (MA1-26196) in ICC/IF

产品信息

MA1-26196

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

免疫组织化学（悬浮细胞） (IHC (Free))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID